TNP1基因多态性与四川地区男性不育的相关性研究

Sing-Nucleotide polymorphisms analyses of TNP1 genes of male Infertility in Sichuan Han Populations

目的探讨TNP1基因单核苷酸多态性(single nucleotide polymorphism,SNP)与中国四川地区男性不育的相关性。方法应用DNA测序技术分别对325例男性不育患者(包括166例非梗阻性无精子症和159例少精子症,病例组)和194例已生育的男性人群(对照组)TNP1基因的3个SNP位点(rs1179733、rs1179734、rs1179735)进行检测。分析比较基因型、等位基因及单体型频率在患者组和对照组中的分布特点。结果 TNP1基因的3个多态性位点的基因型及等位基因频率在病例组与对照组间无显著性差异(P>0.05),进一步进行单体型分析,其频率分布在两组中也无显著性差异(P>0.05)。结论 TNP1基因rs1179733、rs1179734、rs1179735多态性与四川地区男性不育无相关性。

Objective： To study the association between the single nucleotide polymorphisms （SNPs） of transition nuclear protein 1 gene （TNP1） and male infertility in Sichuan Han population . Methods ： A total of 325 cases with male infertility were collected （case group）, including 166 non- obstructive azoospermia cases and 159 oligozoospermia cases, and 194 cases with normal fertility were collected as the control. The genotypes of two SNPs rs1179733, rs1179734 and rs1179735 were detected by DNA sequencing technique. The frequencies of genotype, allele and haplotype were analyzed. Results： Between case group and control group, there was no significant difference in the allele or genotype frequencies of the three SNPs （ P 〉 0. 05） and no significant difference in the hap- lotype frequencies of the three sites （P 〉0.05）. Conclusion： The TNP1 rs1179733, rs1179734 and rs1179735 SNPs are not correla- ted with male infertility in Sichuan Han population.