高效液相色谱法测定己酮可可碱肠溶片中己酮可可碱含量

Content determination of pentoxifylline in pentoxifylline enteric-coated tablets by HPLC

目的：建立测定己酮可可碱肠溶片中己酮可可碱含量的方法。方法采用高效液相色谱法测定己酮可可碱含量。色谱柱：COSMILC18柱（4.6 mm×250.0 mm，5.0μm）；检测波长：272 nm；流动相：甲醇-0.544%磷酸二氢钾溶液（48∶52）；柱温：30℃；流速：1.0 mL/min；进样量：20μL。结果己酮可可碱质量浓度为60.25～140.59μg/mL时峰面积与浓度呈良好的线性关系（R2=1.000）。3批样品的平均含量分别为99.91%、99.95%、99.89%。结论高效液相色谱方法专属性强、方便、快捷，适用于测定己酮可可碱肠溶片中己酮可可碱的含量。

Objective To establish a method for the content determination of pentoxifylline in pentoxifylline enteric-coated tablets. Methods High-performance liquid chromatography （HPLC） was used to determine the content of pentoxifylline. The chromatographic column was COSMILC18（4.6 mm×250.0 mm,5.0μm）;the detection wavelength was 272 nm;the mobile phase was methanol-0.544%monopotassium phosphate（48∶52）;the column temperature was 30℃;the flow velocity was 1.0 mL/min, and the injection volumn was 20μL. Results When the concentrations of pentoxifylline was in the range of 60.25-140.59μg/mL, the peak area had good linear relationship with concentration（R2=1.000）. The average contents of pentoxifylline in three batches of samples were 99.91%,99.95%,99.89%respectively. Conclusion The HPLC is specific,convenient and fast,it is suitable for content determination of drugs in pentoxifylline enteric-coated tablets.