宫颈鳞癌组织中p21和VEGF蛋白表达的实验研究

Experimental study on p21 protein and VEGF protein expressions in cervical squamous cell carcinoma

目的:探讨子宫颈鳞癌组织中p21和VEGF蛋白的表达及其与临床病理特征的关系。方法:采用免疫组织化学技术,检测56例子宫颈鳞癌、24例宫颈原位癌和16例癌旁正常宫颈上皮中p21和VEGF的表达,分析其表达与宫颈鳞癌的临床分期、病理类型及有无淋巴结转移的关系。结果:p21在宫颈鳞癌、原位癌和癌旁正常宫颈上皮中的阳性表达率分别为51.79%、83.33%、93.75%;VEGF为82.14%、58.33%、31.25%。p21和VEGF在癌旁正常宫颈上皮组与鳞癌组及原位癌组相比较差异均有统计学意义(P<0.01)。p21和VEGF的阳性表达率与临床分期、病理类型及有无淋巴结转移均有明显相关性(P<0.01);p21和VEGF在宫颈鳞癌的表达中呈负相关(P<0.01)。结论:宫颈鳞癌组织中p21蛋白表达水平的降低和VEGF蛋白表达水平的上调,可能参与宫颈鳞癌的浸润和转移,两者的联合监测对宫颈鳞癌的诊断、临床分期、预后有指导作用。

Objective： To explore the relationship between p21 protein and VEGF protein expressions and clinicopathogical charac- teristics of cervical squamous cell carcinoma. Methods： Immunohistochemical method was used to detect the expression levels of p21 and VEGF in 56 case with cervical squamous cell carcinoma, 24 cases with cervical carcinoma in situ and 16 cases with normal cervical epitheli- um （NCE） adjacent to carcinoma. The relationship between their expression levels and clinical stages, pathological types and lymph node metastasis or not was analyzed. Results： The positive expression rates of P21 in cervical squamous cell carcinoma, cervical carcinoma in situ and NCE adjacent to carcinoma were 51.79% , 83.33% and 93.75%, respectively; the positive expression rates of VEGF in cervical squa- mous cell carcinoma, cervical carcinoma in situ and NCE adjacent to carcinoma were 82. 14% , 58.33% and 31.25% , respectively. There were statistically significant differences in positive expression rates of P21 and VEGF between NCE adjacent to carcinoma and cervical squa- mous cell carcinoma, cervical carcinoma in situ （P 〈 0. 01 ） . The positive expression rates of P21 and VEGF were significantly correlated with clinical stages, histological types and lymph node metastasis （P 〈 0. 01 ） . There was a negative correlation between p21 expression and VEGF expression in cervical squamous cell carcinoma （P 〈 0. 01 ） . Conclusion： Down - regulation of p21 protein and up - regulation of VEGF in cervical squamous cell carcinoma may be involved in the invasion and metastasis of cervical squamous cell carcinoma, joint monito- ring of p21 and VEGF can guide diagnosis, clinical staging and prognosis of cervical squamous cell carcinoma.