HPVL1壳蛋白在宫颈脱落细胞及宫颈组织中的表达及意义

Expressions and significances of HPV L1 capsid protein in cervical exfoliated cells and cervical tissue

目的:探讨HPVL1壳蛋白在宫颈脱落细胞及宫颈组织中的表达及临床意义。方法:随机选取来医院就诊患者的宫颈脱落细胞标本782例用免疫细胞化学法检测HPVL1壳蛋白的表达,用免疫组织化学方法检测224例宫颈病变组织中HPVL1壳蛋白的表达。结果:HPVL1壳蛋白在NLIM、ASCUS、ASCU-H、LSIM、HSIM中的阳性表达率分别为49.24%(130/264)、43.90%(72/164)、20.54%(23/122)、18.18%(24/132)、15.45%(17/110)。HPVL1壳蛋白在慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ中的阳性表达率分别为42.86%(24/56)、36.11%(26/72)、15.63%(10/64)、6.25%(2/32)。各组织学分级患者HPVL1衣壳蛋白的阳性率差异有统计学意义(P=0.00)。结论:HPVL1壳蛋白在宫颈脱落细胞及宫颈病变组织中的表达率随病变程度的加重均呈下降趋势。

Objective： To explore the expressions and significances of HPV L1 capsid protein in cervical exfoliated cells and cervi- cal tissue. Methods： The expression of HPV L1 capsid protein in cervical exfoliated cells of 782 patients randomly selected from the hospital was detected by immunocytochemical method, and the expression of HPV L1 capsid protein in cervical lesion specimens of 224 patients was detected by immunocytoehemical method. Results： The positive expression rates of HPV L1 capsid protein in NLIM, ASCUS, ASCU - H, LSIM and HSIM were 49.24% （130/264）, 43.90% （72/164）, 20.54% （23/122）, 18. 18% （24/132） and 15.45% （17/110）, re- spectively. The positive expression rates of HPV L1 capsid protein in chronic cervicitis, CIN Ⅰ , CIN Ⅱ and CIN Ⅲ were 42. 86% （24/56）, 36. 11% （26/72）, 15.63% （10/64） and 6. 25% （2/32）, respectively. There was statistically significantly difference in positive expres- sion rate of HPV L1 eapsid protein among patients of different histological grades （P = 0. 00） . Conclusion： The expression rates of HPV L1 eapsid protein in cervical exfoliated cells and cervical tissue show decreasing trend with aggravation of the disease.