上调胰岛素样生长因子结合蛋白相关蛋白1对子宫内膜癌细胞系HEC-1A生物学功能的影响

Effect of up-regulation of insulin like growth factor binding protein-related protein 1 on biological function of endometrial cancer-1A cell line

目的:探讨上调子宫内膜癌细胞系HEC-1A中胰岛素样生长因子结合蛋白相关蛋白1的表达对子宫内膜癌细胞系HEC-1A增殖,凋亡及细胞周期的影响。方法:采用脂质体介导重组质粒转染HEC-1A细胞,按不同处理分为实验组(转染pEX-2-IGFBP7)、阴性对照组(转染pEX-2-Empty)、空白对照组(只加转染试剂),应用qRT-PCR技术检测各组细胞中IGFBP-rP1mRNA的表达水平,利用蛋白印迹法(Western Blot)测定各组IGFBP-rP1蛋白表达。采用四甲基偶氮唑蓝(MTT)法检测各组细胞系增殖抑制率,采用流式细胞仪检测各组凋亡率及细胞周期变化。结果:实验组、阴性对照组和空白对照组HEC-1A细胞中IGFBP-rP1mRNA的相对拷贝量分别为2.699±0.293,1.296±0.169,1.031±0.301,实验组明显高于阴性对照组(P=0.001)及空白对照组(P=0.000)。实验组IGFBP-rP1蛋白相对表达量(1.126±0.074)与阴性对照组(0.889±0.040,P=0.006)及空白对照组(0.884±0.043,P=0.005)中相比,差异有统计学意义。实验组24、48和72 h增殖抑制率(0.373±0.054,0.399±0.047,0.380±0.053)与阴性对照组(0.036±0.006,0.040±0.005,0.0334±0.006)和空白对照组(0.027±0.003,0.032±0.002,0.024±0.002)相比,差异均有统计学意义(P<0.01)。而阴性对照组与空白对照组增殖抑制率相比,差异无统计学意义(P>0.05)。转染后24 h,实验组凋亡率为(20.667±2.055)%,与阴性对照组[(3.967±0.351)%]和空白对照组[(4.967±0.252)%]相比,差异有统计学意义(P<0.01);实验组S+G2/M期细胞的比例为(30.980±1.461)%,明显低于阴性对照组[(38.797%±2.419)%]和空白对照组[(37.800%±0.350)%],差异有统计学意义(P<0.01)。结论:上调人子宫内膜癌细胞HEC-1A中IGFBP-rP1的表达可以抑制该细胞系的增殖,促进其凋亡,阻滞细胞周期。

Objective： To explore the effect of up - regulation of insulin like growth factor binding protein - related protein 1 （ IG- FBP- rPl ） on proliferation, apoptosis and cell cycle of endometrial cancer- 1A （HEC -1A） cell line. Methods： Plasmid- mediated recombinant plasmid was transfected into HEC - 1A cells, then the cells were divided into experimental group （ transfected with plasmid pEX - 2 -IGFBP7 ） , negative control group （transfected with plasmid pEX -2 -Empty） and blank control group （only adding reagent of transfec- tion） . The expressions of IGFBP- rP1 mRNA in the three groups were detected by qRT- PCR, the expressions of IGFBP- rP1 protein in the three groups were detected by Western Blot. MTT method was used to detect the inhibition rates of proliferation of HEC - 1A cells in the three groups, flow cytometry was used to detect the changes of apoptotic rates and cell cycles in the three groups. Results： The relative copy numbers of IGFBP -rP1 mRNA in HEC - 1A cells of experimental group, negative control group and blank control group were （2. 699±0. 293）, （1. 296 ±0. 169） and （1. 031 ±0. 301）, respectively, the relative copy number of IGFBP -rP1 mRNA in HEC - 1A cells of ex- perimental group was statistically significantly higher than those in negative control group （P = 0. 001 ） and blank control group （P = 0. 000 ） The relative expression levels of IGFBP - rP1 protein in HEC - 1A cells of experimental group, negative control group and blank control group were （ 1. 126 ±0. 074） , （0. 889±0. 040） and （0. 884 ±0. 043） , respectively, there was statistically significant difference in the relative expression level of IGFBP- rP1 protein in HEC -1A cells between experimental group and negative control group （P = 0. 006） , blank control group （P = 0. 005） . The inhibition rates of proliferation of HEC - 1A cells at 24, 48 and 72 hours in experimental group were （0. 373 ±0. 054）, （0. 399 ±0. 047） and （0. 380±0. 053）, compared with negative control group [ （0. 036 ±0. 006）, （0. 040 ±0. 005） and （0. 0334 ± 0. 006） ] and blank control group [ （0. 027 ± 0. 003 ）, （ 0. 032± 0. 002 ） and （ 0. 024 ± 0. 002 ） ], there were statistically significant differences （P 〈0. 01 ）, but there was no statistically significant difference between blank control group and blank control group （ P 〉 0. 05 ） . At 24 hours after transfection, the apoptotic rate in experimental group was （ 20. 667 ±2. 055 ） %, compared with negative control group [ （3. 967 ±0. 351 ） % ] and blank control group [ （4. 967 ±0. 252 ） % ], there was statistically significant difference （ P 〈 0. 01 ） ; the ratio of S ＋ G2 cell/M phase cell in experimental groups was （30. 980 ± 1. 461 ）%, which was statistically significantly lower than those in negative control group [ （38. 797% ± 2. 419） % ] and blank control group [ （37. 800% ± 0. 350） % ] （P 〈 0. 01 ） . Conclu- sion： Upregulating IGFBP- rP1 expression in HEC -1A cells can inhibit cell proliferation, promote cell apoptosis and arrest cell cycle.