里氏木霉Rut-C30产纤维素酶培养基优化及其酶解特性

Optimization of the Fermentation Medium for Cellulase Production from Trichoderma reesei Rut-C30 and Its Enzymatic Hydrolysis Characteristics

以廉价的工业纤维素诱导里氏木霉Rut-C30产纤维素酶,并对液体深层发酵培养基进行优化,采用响应面中心组合设计,以滤纸酶活为响应值,考察工业纤维素、麦麸、大豆粉浓度对纤维素酶活的影响.结果表明,优化后的培养基组成为:工业纤维素35.62 g/L、麦麸19.37 g/L、大豆粉38.49 g/L,该条件下滤纸酶活达9.13 IU/mL,比优化前提高了72.26%,葡萄糖苷酶酶活提高了80.39%.在121℃下用2%NaOH对玉米秸秆预处理45 min,物料中纤维素含量达64.94%,用该粗酶液酶解后酶解得率为94.68%.

Cheap industrial cellulose was taken as substrate for cellulase production from Trichoderma. reesei Rut-C30. The five-level three-variable central composite design implemented in the package of response surface methodology was adopted to evaluate the effects of industrial cellulose, wheat bran and soy flour on filter paper activity of （FPA） of cellulose. The results show that the optimal formulation of fermentation medium was determined as follows： industrial cellulose 35.62 g/L, wheat bran 19.37 g/L and soy flour 38.49 g/L. Thus, FPA achieved 9.13 IU/mL, which was increased by 72.26% over that without optimization, and the glucosidase activity was also increased by 80.39%. This crude cellulase solution was applied to obtain a hydrolysis yield of 94.68% in the hydrolysis of corn stalk which was pretreated by 2% NaOH for 45 min under 121℃, and the cellulose content of 64.94%.