硒代蛋氨酸遗传毒性的研究

STUDY ON THE GENETIC TOXICITY OF SELENOMETHIONINE

目的探讨硒代蛋氨酸对小鼠微核及精子畸变的影响及硒代蛋氨酸是否具有基因水平的诱变性,研究硒代蛋氨酸的遗传毒性。方法设三个硒代蛋氨酸剂量实验组,一个阴性对照组和一个阳性对照组。分别进行雌雄小鼠骨髓微核试验和雄性小鼠精子畸变试验。选用TA97、TA98、TA100、TA102四种菌进行硒代蛋氨酸的Ames试验。结果微核实验阴性对照组的微核率为1.7%,阳性对照为6.92%,在0.06mg/mL剂量时微核率为3.2%,结果为阳性;在0.03 mg/ml剂量时微核率为2.5%,结果为阴性。精子畸变实验阴性对照组的精子畸变率为1.22%,阳性对照为4.36%,在0.06 mg/ml剂量时精子畸变率为2.24%%,结果为阳性;在0.03 mg/ml剂量时精子畸变率为1.86%,结果为阴性。Ames试验结果为硒代蛋氨酸对TA97、TA98、TA100、TA102四种菌株均无致突变活性。结论在实验剂量范围内,硒代蛋氨酸不具有基因水平的诱变性,可以作为食品添加剂。通过对硒代蛋氨酸遗传毒性的研究,从小鼠骨髓细胞微核试验和小鼠精子畸变试验都可得出试验在大于0.06 mg/ml剂量时结果为阳性;试验在小于0.03 mg/ml剂量时结果为阴性。硒代蛋氨酸作为一种补充硒元素的有效途径,本实验说明低浓度的硒代蛋氨酸可用于富硒食品的生产,而高浓度的硒代蛋氨酸对生物体有一定的损伤作用。

Objective To explore the effects of selenomethionine on micronucleus and aberration of sperm in mice, and to study the gene mutagenicity and genotoxicity of selenomethionine. Methods KM mice of 6-8 weeks old were randomly divided into three selenomethionine groups, a negative control group and a positive control group, respectively. Bone marrow micronucleus test in male and female and sperm aberration test in male mice were carried out. TA97, TA98, TA100, TA102 bacteria were selected in Ames test. Results Micronucleus rate of negative control group was 1.7%, 6.92%, for positive control In 0.06 mg/ml dose group, the micronucleus rate was 3.2%, and defined as positive; In 0.03 mg/ml dose group, micronucleus rate was 2.5%, and defined as negative. Sperm aberration in negative control group was 1.22%, In positive control. 4.36%, and in 0.06 mg/ml group 2.24%. The result was defined as positive. In 0.03 mg/ml dose group, sperm abnormality rate was 1.86%, and defined as negative. Selenomethionine had no mutagenic activity for TA97, TA98, TA100, TA102 bacteria in Ames test. Conclusion In the tested dose range, selenomethionine has no gene mutagenicity, In genotoxicity studies, at the dose more than 0.06 mg/ml is positive; and negative at the dose less than 0.03 mg/ml. This experiment shows that selenomethionine at low concentrations is suitable for food selenium fortification, while at high concentrations, selenomethionine is harmful.