摘要
目的:建立高效液相色谱法测定补。肾强身片中原儿茶酸的含量方法。方法:采用色谱柱为AgilentHC—C18(4.6mm×250mm,5μm);流动相:乙腈-1%冰醋酸(3.5:96.5);柱温为30℃;流速:1.0m1.min-1;检测波长:260nm;进样量:101.d。结果:原儿茶酸回归方程Y--3081.5151X-0.9753,r=0.9998(n=5),浓度在0.05888~0.35328gig.ml-1范围内线性关系良好,平均回收率97.7%,RSD为0.96%。结论:HPLC法准确、快速,简便可靠,可用于补肾强身片中原儿茶酸的含量测定。
Objective: To establish an HPLC method for determination of Protocateehuic acid in Bushenqiangshen tablets. Meth- ods: The Agilent HC - C18 column (4. 6mm ×250mm, 5μm) was used at a column temperature of 30℃. The mobile phase consis- ted of acetonitrile - 1% glacial acetic acid in water (3.5: 96. 5) and detected with UV 260nm. The flow rate was 1.0mL · min-1 and the injected volume was 10μL. Results: A good linear concentration range for Protocatechuic acid was 0. 05888 -- 0. 35328μg. mL-1 ( r = 0. 9998 , n = 5). The average recoveries was 97.7% with RSD of 0. 96% . Conclusion : The method is rapid, simpie and accu- rate , which can be used for the quality control of Protocatechuic acid of Bushenqiangshen tablets.
出处
《中国民族民间医药》
2014年第9期13-14,共2页
Chinese Journal of Ethnomedicine and Ethnopharmacy