外周血黑色素瘤抗原基因表达与胃癌转移及预后的关系

Expression of MAGE genes peripheral blood of gastric cancer patients and its relation to lymph node metastasis and prognosis

目的 检测胃癌患者外周血中黑色素瘤抗原A1（MAGEA1）和黑色素瘤抗原A3（MAGEA3）基因表达,结合患者术后随访情况,分析MAGE基因表达与胃癌转移及预后的关系.方法 术前采集40例胃癌患者及20例健康人外周血,用RT-PCR方法检测外周血单核细胞（PBMC）中MAGEA1和MAGEA3基因mRNA.用RT-PCR方法检测胃癌组织中上述MAGE基因的表达,同时检测上述标本CEA表达.结果 47.5％（19/40）和25.0％（10/40）胃癌患者外周血PBMC中分别检测到MAGEA1和MAGEA3基因mRNA,而相应的胃癌组织中MAGEA1和MAGEA3基因表达率分别为60.0％ （24/40）和30.0％（12/40）,相应的癌旁胃组织均不表达,其中有4例外周血两者均表达.在62.5％ （25/40）的胃癌患者外周血单核细胞（PBMC）中至少检测到一种MAGE基因mRNA;上述患者外周血PBMC中CEA基因mRNA的检出率为65.0％ （26/40）,20例非肿瘤疾病患者的PBMC中均未检出MAGE基因mRNA.胃癌患者PBMC中MAGE基因mRNA的检出率与肿瘤淋巴结转移、TNM分期相关,而与肿瘤分化程度、浸润深度、肿瘤大小、远处转移无关.结论 胃癌患者外周血MAGE基因表达与肿瘤淋巴结转移、TNM分期密切相关,表达阳性的患者预后差.

Objective In the detection of peripheral blood in patients with gastric cancer the melanoma antigen A1 （MAGEA1） and the melanoma antigen A3 （MAGEA3） gene expression,combined with the conditions of patients with postoperative follow-up analysis MAGE gene expression and the relationship between gastric cancer metastasis and prognosis.Methods Peripheral blood was obtained from 40 patients with gastric carcinoma and 20 patients with benign diseases.The mRNA of the MAGEA1 and MAGEA3 genes in the peripheral blood mononuclear cells（PBMC） was detected by RT-PCR,meanwhile the MAGEA1 and MAGEA3 transcripts in gastric carcinoma tissues and corresponding nomal tissue were detected.In contrast with CEA gene,we detected its expression by nested RT-PCR.Results Of the 40 gastric carcinoma patients,MAGEA1 and MAGEA3 mRNA were positive in 47.5% （17/40） and 25.0% （10/40） of PBMC,respectively,and in 65.0% （26/40） and 30.0% （12/40） of gastric carcinoma tissues,the corresponding paracancerous gastric tissues were not expressed,of which 4 cases of peripheral blood both expression.In the PBMC of 40 gastric carcinoma patients,62.5% （25/40） samples were detected to express at least one type of MAGE mRNA,the detection of CEA gene mRNA in peripheral blood of the patients in PBMCwas 65.0% （26/40）,20 patients with non neoplastic diseases of PBMC were not detected in the MAGE gene mRNA.There were 22.5% （9/40） of 40 gastric carcinoma patients with CEA gene expression in PBMC.The detection rate of the MAGE gene of mRNA PBMC in patients with gastric cancer was not related with the lymph node metastasis,TNM stage,and the degree of tumor differentiation,depth of invasion,tumor size,distant metastasis.Conclusion Gastric cancer patients with peripheral blood MAGE gene expression is closely related to the tumor lymph node metastasis,TNM staging,express positive patients with poor prognosis.