米非司酮调控乳腺癌细胞凋亡的研究

Effect of mifepristone on the apoptosis of human breast cancer cell line T47D in vitro

目的:研究米非司酮促进乳腺癌T47D细胞凋亡的作用。方法:根据米非司酮的处理浓度分为0.25μmol/L组、2.5μmol/L组、25μmol/L组和50μmol/L组,各组分别加入相应浓度米非司酮处理人乳腺癌T47D细胞2d后,采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法检测细胞凋亡,在荧光显微镜下观察绿色荧光细胞数量和荧光强度,采用膜联蛋白V-异硫氰酸荧光素/碘化吡啶法在流式细胞仪上检测凋亡细胞百分率。结果:25μmol/L组和50μmol/L组与对照组比较,阳性绿色荧光细胞的数量更多,荧光强度更强;25μmol/L组和50μmol/L组的凋亡细胞百分比(分别为16.1±3.5%和17.2±3.8%)高于对照组(5.1±2.3%),差异有统计学意义(分别为P=0.010和P=0.009);0.25μmol/L组和2.5μmol/L组的凋亡细胞百分比与对照组比较差异无统计学意义(分别为P=0.818和P=0.884)。结论:米非司酮能够促进乳腺癌T47D细胞的凋亡,且随着米非司酮剂量的升高,凋亡细胞数量增加。

Objective： To investigate the promoting effect of mifepristone on the apoptosis of human breast cancer cells T47D in vitro. Methods： T47D cells were treated by different concentrations （0.25, 2.5, 25 and 50μmol/L） of mife- pristone. Then the apoptosis of these cells was examined by TUNEL assay under the microscope on day 2. The apopto- sis was also examined by Annexin V--FITC assay. Results： Compared with the control group, the number and intensi- ties of the TUNEL--positive cells in 25μmol/L group and 50μmol/L group were significantly higher and stronger. The apoptosis rates of 25μmol/L group （16.1±3.5%, P=0.010） and 50μmol/L group （17.2±3.8%, P=0.009） were sig- nificantly higher than that of the control group （5.1 ± 2.3 % ）. There were no significant differences between the control group and 0.25μmol/L group （P=0.818） as well as 2.5μmol/L group （P =0.884）. Conclusion： Mifepristone can in- duce the apoptosis of T47D cells in a dose--dependent manner.