家蚕卵线粒体DNA限制性内切酶长度多态性研究

RESTRICTION ENDONUCLEASE FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF MITOCHONDRIA DNA IN EGGS OF BOMBYX MORI

利用 2 9种限制性内切酶对 34个不同品种家蚕卵mtDNA进行酶切分析 ,发现一化、二化品种与多化品种的HaeⅢ酶切图谱有差异 ,但不同的地理品种间没有出现酶切多态性现象。另外 ,一些家蚕品种的受精卵和非受精卵mtDNA在BglⅠ和PstⅠ酶切下 ,呈现不同的酶切带型。同时 。

The restriction endonuclease maps of mitochondria DNA (mt DNA) from the eggs of 34 domestic silkworm ( Bombyx mori) strains were analyzed through 29 different restriction endonucleases.mtDNA of multivoltine strains showed different RFLP (restriction endonuclease length polymorphism) from that of monovoltine and bivoltine strains,but no RFLP was shown to exist between different geographical strains.RFLP was also observed between fertilized and unfertilized eggs of the same breed in their mtDNA.Restriction endonuclease maps with moderate preciseness were constructed of domestic silkworm.