大蒜提取物S-烯丙基半胱氨酸对人食管鳞癌TE-13细胞株增殖和凋亡作用的研究

Effect of Garlic Derivative S-allylcysteine(SAC) on Proliferation and Apoptosis of Esophageal Squamous Cell Carcinoma TE-13 Cell Line

[目的]研究S-烯丙基半胱氨酸(SAC)对人食管鳞癌细胞株TE-13增殖、凋亡等生物学特性的影响。[方法]MTT比色法和细胞克隆形成实验检测SAC作用于人食管鳞癌细胞株TE-13的增殖活性,流式细胞术分析肿瘤细胞的凋亡,Western Blot检测细胞内STAT3、Mcl-1、Survivin蛋白的表达。[结果]SAC明显抑制TE-13细胞的增殖率和克隆形成率,并且具有时间和浓度依赖性。进一步研究发现SAC作用于细胞后通过抑制STAT3、Mcl-1和Survivin蛋白的表达,活化Active-caspase 3蛋白的表达从而诱导细胞凋亡和坏死。[结论]S-烯丙基半胱氨酸通过抑制STAT3、Mcl-1和Survivin的表达,进而抑制TE-13细胞的增殖,诱导其凋亡。

[Purpose] To investigate the effect of S-allylcysteine （SAC） on proliferation and apoptosis of human esophageal squamous cell carcinoma TE-13 cell line. [ Methods ] Cell prolifera- tion was detected by MTF and colony-forming assays. Cell apoptosis was detected by Annexin V/PI staining with flow cytometry. The expression of STAT3,Mcl-1 and Survivin was detected by Western Blot assay. [Results] The proliferation rate and colony-forming abilities of TE-13 cells were suppressed by SAC in dose-dependent and time-dependent manners. Moreover, SAC significantly induced apoptosis and necrosis of TE-13 cells through suppressing STAT3,Mcl-1, Survivin as well as activating caspase 3. [Conclusion] SAC inhibits cell proliferation and in- duces apoptosis by inhibiting STAT3 signal transduction in human esophageal squamous cell carcinomas TE-13 cell line.