摘要
90B4 2 2菌株的培养及最大菌落数产生的发酵模拟生产工艺条件 ,正交试验得到最适培养基碳、氮源组成为 ( % ) :黄豆饼粉 1.0 ,淀粉 1.5,酵母粉 0 .8;最适生长温度为 30℃ ;pH7.0 ,接种量 4 % ,在 2 50mL三角瓶中装 70mL培养基 ,2 0 0r/min摇床上培养 35h ,菌落数为 6.69×10 9CFU/mL ;上罐 ( 1～ 2t)发酵 2 2h ,终点总的菌落数为 ( 1.85～ 2 .50 )× 10 10 CFU/mL。
The simulated production conditions of bacterial wilt antagonistic strain 90B4 2 2 were studied through culture with different steps and orthogonal test of different medium compositions. The results are summarized as follows. The optimum medium composition is (%): soy bean meal(1.0)+starch(1.5)+yeast powder(0.8). The optimum growth temperature is 30℃. When the strain is cultured for 35 hours on a 200r/min shaker with 70mL medium in 250mL flask at 7.0pH and 4% inoculation level, the total bacterial count is 6.69×10 9CFU/mL. When the fermentation is done in 1～2 ton fermenters, the final total bacterial counts are 1.85～2.50×10 10 CFU/mL.
出处
《上海农业学报》
CSCD
2001年第1期70-73,共4页
Acta Agriculturae Shanghai
基金
上海市科委科技发展基金项目
