原子荧光光谱法测定生物材料中总砷

Determination of total arsenic in biological materials with atomic fluorescence spectrometry

目的建立应用PF6—2型双道原子荧光光谱仪测定生物材料中总砷的方法。方法样品经湿法消解或微波消解后，用原子荧光光度法测定其砷含量。结果检出限为0．50ng／ml，在0～50mg／ml内线性关系良好，相关系数r为0．9993。与现行测砷的方法相比，测定结果差异无统计学意义（t=1．412，P〉0．05）。微波消解的精密度相对较好，方法的RSD在2％～4％之间，加标回收率在91．O％～104．7％之间。结论原子荧光光谱法分析速度快，检出限低，精密度和准确度好，适合生物材料中总砷的测定。

Objective To establish a method for measuring total arsenic in biological materials with double channel atomic fluorescence spectrometer of type PF6 -2. Methods Mercury in the sample was measured with atomic fluorescence spec- trometry after wet digestion or microwave digestion processes. Results The detection limit was 0. 5 ng/ml of a good linear relationship between 0 - 50 mg/ml, the correlation coefficient was 0. 999 3. There was no significant difference in results of the determination between this method and the current method for measuring arsenic. The precision of microwave digestion was better. The precision of the RSD was 2% -4%, the recovery of standard addition was 91.0% - 104.7%. Conclusion The speed of analysis of atomic fluorescence spectrometry is fast, detection limit is low, accuracy and precision is high, and it is a good method for the determination of total arsenic in biological materials.