碱处理对人血白蛋白分子的影响

Influence of alkali treatment on human serum albumin molecules

目的 初探不同碱处理条件对人血白蛋白（human serum albumin,HSA）分子的影响.方法 在保持pH 10.0±0.2的条件下,以不同钠离子浓度和辛酸钠浓度处理白蛋白,通过圆二色谱和高效液相色谱对碱处理的HSA进行检测,分析不同处理条件对HSA分子的影响.结果 在保持pH 10.0 ±0.2且辛酸钠浓度为0.16 mmol/g的条件下,以不同钠离子浓度（40～ 800 mmol/L）处理获得的单体和双体HSA含量均＞96％,而且碱处理HSA的圆二色谱峰值（6.7 ～8.2 cm）与合格HSA（7.5 ～8.9 cm）相近,峰值位置没有偏移.在保持pH 10.0±0.2且钠离子浓度为40 mmol/L的条件下,以不同辛酸钠浓度（0.16～ 1.00 mmol/g）处理获得的的单体和双体HSA含量均＞97％,碱处理HSA的圆二色谱峰值（6.1 ～8.2 cm）与合格HSA（7.5 ～ 8.9 cm）相近,峰值位置没有偏移.结论 在一定条件下对HSA进行碱处理不会对其结构产生明显影响.

Objective To preliminarily study the influence of different alkali treatment conditions on human serum albumin （HSA） molecules.Methods HSAs were treated with different sodium ion concentrations and different sodium caprylate concentrations at pH 10.0 ± 0.2.Alkali treated HSA was detected by circular dichroism and high performance liquid chromatography to analyse the influence of different treatment conditions on HSA molecules.Results Under the condition of pH 10.0 ± 0.2 and 0.16 mmol/g sodium caprylate,contents of monomer and dimer HSAs obtained by treatment of different sodium ion concentrations （40-800 mmol/L） were all 〉96%,and peak value of circular dichroism of alkali treated HSA （6.7-8.2 cm） was similar to that of the qualified HSA （7.5-8.9 cm） and peak value position did not migrate.Under the condition of pH 10.0 ± 0.2 and 40 mmol/L sodium ion,contents of monomer and dimer HSAs obtained by treatment of different sodium caprylate concentrations （0.16-1.00 mmol/g） were all 〉97%,and peak value of circular dichroism of alkali treated HSA （6.1-8.2 cm） was similar to that of the qualified HSA （7.5-8.9 cm） and peak value position did not migrate.Conclusion HSA structure does not fundamentally change when alkali treatment for HSA is carried out under certain conditions.