人血浆载脂蛋白AⅠ的纯化

Purification of apolipoprotein AⅠ from human plasma

目的 初探大规模纯化人血浆载脂蛋白AⅠ（apolipoprotein A Ⅰ,ApoA Ⅰ）的低成本生产工艺.方法 以人血浆组分Ⅳ为原料,通过等电点沉淀、阴离子层析、低温乙醇沉淀和超滤等步骤纯化ApoA Ⅰ蛋白,并放大实验室纯化方法来纯化ApoA Ⅰ.结果 放大纯化方法生产的ApoA Ⅰ的得率和纯度分别为59.0％和85.6％,经相关方法验证为人血浆ApoAⅠ.结论 实验室方法放大的纯化工艺可纯化ApoA Ⅰ.

Objective To preliminarily explore a low-cost production process of purifying apolipoprotein A I （ ApoA I ） from human plasma in large scale. Methods ApoA I was purified from human plasma fraction IV through isoelectric precipitation, anion exchange chromatography, ethanol precipitation and uhrafiltration steps, and the laboratory purification method was amplified to purify ApoA I . Results The yield and purity of ApoA I produced by amplified purification method were 59.0% and 85.6% respectively, and ApoA I was verified with relative methods. Conclusion The purification process amplified from laboratory method can purify ApoA I .