姜黄素对非酒精性脂肪肝细胞胆固醇代谢的影响

Effect of Curcumin on Cholesterol Metabolism of Nonalcoholic Fatty Liver Disease Cells

[目的]探讨姜黄素对体外诱导的非酒精性脂肪肝模型细胞胆固醇代谢的影响及其机制。[方法]采用油酸诱导法建立非酒精性脂肪肝细胞模型,并用姜黄素进行处理,油红O染色观察细胞内脂质累积情况,酶法检测细胞内甘油三酯、游离胆固醇和总胆固醇含量,定量PCR检测SR-BΙmRNA和HMGCR mRNA的表达。[结果]30μg·mL-1油酸可成功建立非酒精性脂肪肝细胞模型,姜黄素干预后,脂肪肝细胞中甘油三酯和游离胆固醇减少,细胞内脂质积累减少,而总胆固醇无显著变化。姜黄素还能降低HMGCR mRNA表达,而增加SR-BⅠmRNA表达。[结论]姜黄素能够降低脂肪肝细胞游离胆固醇,其机制与其抑制内源性胆固醇的生物合成,促进外源胆固醇进入肝细胞进行代谢有关。

[Objective]To study the effect of curcumin on the cholesterol metabolism of nonalcoholic fatty liver disease（NAFLD） cells model induced in vitro and its potential mechanism. [ Methods]The cellmodel of nonalcoholic fatty liver disease（NAFLD） was established by oleic acid and treated with curcumin. The method of oil red O staining was used to observe accumulation of intracellular lipid while the intracellular content of TG, FC and TC was detected by enzymatic method. Meanwhile, the mRNA levels of SR-BΙand HMGCR were detected with qPCR.[ Results] The NAFLD cellmodel was successful y established by culturing with 30 μg·mL^-1 oleic acid. After curcumin intervention, TG, FC and intracellular lipid accumulation levels were significantly reduced in NAFLD cellmodel. Meanwhile, curcumin can reduce HMGCR mRNA expression and raise SR-BΙ mRNA expression. [Conclusion] Curcumin can decrease FC level in NAFLD cellmodel and the mechanism might be related with its capacity of restraining endogenous cholesterol biosynthesis and promoting foreign cholesterol transfer into the liver cells for metabolism.