胰蛋白酶生产中的固液分离工艺研究

Solid-liquid separation process in production of trypsin

胰蛋白酶生产中的固液分离对产品的质量和后续处理具有很大影响。在保持酶活的前提下,分别在提取猪胰浆前后用有机试剂去除脂肪,以达到改善提取后固液分离效果的目的。结果表明用有机试剂处理提取前的胰浆效果不理想,而用有机试剂处理提取液效果较佳。其中用三氯乙烯处理提取液,与原工艺相比,离心沉降后的浊度及过滤速度有较大改善。当提取液与三氯乙烯体积比为1∶1,处理时间为1 h时,胰蛋白酶的酶活损失仅为3.22%,浊度从2.52降低到0.57,过滤速度从0.46 mL/min提高到2.11 mL/min。

The solid-liquid separation,which has a great impact on the quality and subsequent product purification,is a critical step in the production of trypsin.In order to improve the effect of solid-liquid separation,several kinds of organic reagents are used to remove the fat before and after the extraction of pancreas.The method involving in dealing with the pancreatic slurry before extraction is not feasible.In contrast,better solid-liquid separation effect can be achieved with pancreatic slurry after extraction treatment.The turbidity and filtration rate of extraction has been greatly changed.The loss of enzyme activity of trypsin is only 3.22％.The turbidity is reduced from 2.52 to 0.57.The filtration rate increases from 0.46 mL/min to 2.11 mL/min when the volume ratio of extraction and trichloroethylene is 1 ∶ 1 and the processing time is 1 hour.This easy-operating method which can be applied to real production may provide an additional 80％ of separation time-saving.Meanwhile,it can also make the cost down by recovery of the solvents.