蓝莓花青素对肥胖大鼠血脂、血清炎性因子及胰岛素敏感性的影响

Effects of blueberry anthocyanidin on dyslipidemia, proinflammatory cytokines and insulin sensitivity in high-fat-diet induced obese rats

目的 观察蓝莓花青素对肥胖大鼠血脂、血清炎性因子及胰岛素敏感性的影响.方法 雄性SD大鼠30只随机数字表法分为对照组（7只,普通饲料喂养）和高脂组（23只,高脂饲料喂养）.6周后,肥胖造模成功的16只大鼠随机均分为肥胖组和花青素组.花青素组予蓝莓花青素200 mg/（kg＆#183;d）灌胃,其余2组予等量蒸馏水灌胃.4周后,空腹采血,全自动生化分析仪测血糖、三酰甘油（TG）、总胆固醇（TC）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）,放射免疫分析法测空腹胰岛素,酶联免疫吸附实验测肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）、超敏C-反应蛋白（hs-CRP）.处死大鼠分离内脏脂肪组织称重,并计算稳态模型评估胰岛素抵抗指数（HOMA-IR）和内脏脂肪比.应用SPSS 19.0统计软件进行数据分析.结果 花青素组血糖[（9.15＆#177;1.10） mmol/L]、TC[（462.5＆#177;81.5） mg/L]、TG[（152.5＆#177;19.1） mg/L]、LDL[（203.7＆#177;31.1） mg/L]、TNF-α[（4.03＆#177;1.03） ng/L]、IL-6[（1.81 ＆#177;0.31） ng/L]、hs-CRP[（106.62＆#177;15.52） ng/L]和HOMA-IR（3.94＆#177;0.78）均较肥胖组[分别为（11.38＆#177;1.50） mmol/L、（566.2＆#177;113.2） mg/L、（192.5＆#177;24.9） mg/L、（253.7＆#177;58.3）mg/L、（5.74＆#177;1.61） ng/L、（3.85＆#177;0.93） ng/L、（132.62＆#177;17.18） ng/L、5.09＆#177;0.90]显著降低,差异均有统计学意义（P均＜0.05）;但仍高于对照组[（7.52＆#177;1.37） mmol/L、（377.1＆#177;71.3） mg/L、（124.2＆#177;26.3）mg/L、（135.7＆#177;38.2） mg/L、（2.41＆#177;0.42） ng/L、（1.12＆#177;0.46） ng/L、（72.28＆#177;12.11） ng/L、2.41＆#177;0.23];而HDL[（126.2 ＆#177;38.1） mg/L]显著高于肥胖组[（95.0＆#177;25.6） mg/L],但仍低于对照组[（165.7＆#177;22.3） mg/L];上述差异均有统计学意义（P均＜0.05）.肥胖组体质量、内脏脂肪比及胰岛素[（439.75＆#177;19.44）g、（4.62＆#177;0.39）％、（10.22＆#177;1.42） mIU/L]与蓝莓花青素组[（431.38＆#177;13.10）g、（4.30 ＆#177;0.37）％、（9.81＆#177;0.98）mIU/L]相比,差异均无统计学意义（P均＞0.05）,但上述指标明显高于对照组[（358.86＆#177;15.32）g、（3.61＆#177;0.45）％、（7.22＆#177;2.06） mIU/L],差异均有统计学意义（P均＜0.05）.肥胖大鼠体质量、内脏脂肪比、TC、TG、LDL、TNF-α、IL-6、hs-CRP、血糖、胰岛素与HOMA-IR呈正相关（r=0.733、0.668、0.602、0.607、0.683、0.728、0.784、0.907、0.743,P均＜0.01）,HDL与HOMA-IR呈负相关（r=-0.581,P＜0.01）.结论 蓝莓花青素可增加肥胖大鼠胰岛素敏感性,这种作用可能与改善血脂紊乱及弱炎症状态有关.

Objective To investigate the effect of blueberry anthocyanidin on serum lipid profiles,proinflammatory cytokines and insulin sensitivity in high-fat-diet induced obese rats.Methods Male Sprague-Dawley rats were randomly grouped to receive a standard chow diet （control group,7 cases） or high-fat-diet （23 cases）.Six weeks later,high-fat-diet induced obese rats were divided into the 2 groups.Then,rats received a standard chow diet,a high-fat-diet,or a high-fat-diet supplemented with anthocyanidin extracted from blueberry at 200 mg/（kg ＆#183; d） for 4 weeks,and were then euthanized.Parameters of body weight,visceral adipose tissue,serum glucose,insulin,total cholesterol（TC）,triglyceride （TG）,low-density lipoprotein （LDL）,high-density lipoprotein （HDL）,tumor necrosis factor-or （TNF-α）,interleukin-6（IL-6）,high sensitive C-reactive protein （hs-CRP） and homeostasis model assessment insulin resistance （HOMR-IR） were measured.Results The levels of glucose [（9.15 ±1.10） mmol/L],TC[（462.5 ± 81.5） mg/L],TG[（152.5 ±19.1） mg/L],LDL[（203.7±31.1） mg/L],TNF-α[（4.03 ± 1.03） ng/L],IL-6[（1.81 ±0.31） ng/L],hs-CRP[（106.62 ± 15.52） ng/L] and HOMA-IR （3.94 ± 0.78）in blueberry anthocyanidin group were lower than those in the obese rats [（11.38 ± 1.50） mmol/L,（566.2 ± 113.2） mg/L,（192.5 ± 24.9） mg/L,（253.7 ± 58.3）mg/L,（5.74 ± 1.61） ng/L,（3.85 ±0.93） ng/L,（132.62 ± 17.18） ng/L,5.09 ±O.90],and the differences were significant（all P 〈 0.05）.But the levels of the parameters in blueberry anthocyanidin group were higher than the control group [（7.52±1.37） mmol/L,（377.1 ±71.3） mg/L,（124.2 ±26.3） rng/L,（135.7 ±38.2） rng/L,（2.41 ±0.42）ng/L,（1.12 ±0.46） ng/L,（72.28 ±12.11） ng/L,2.41 ±0.23],and the differences were significant（all P 〈0.05）.The level of HDL in blueberry anthocyanidin group [（126.2 ± 38.1） mg/L] was higher than that in obese group [（95.0 ±25.6 mg/L）],but the level of HDL in blueberry anthocyanidin group was lower than that in the control group [（165.7 ± 22.3） mg/L],and the differences were significant （all P 〈 0.05）.Body weight [（439.75 ± 19.44） g],visceral adipose tissue [（4.62 ± 0.39） %] and insulin [（10.22 ± 1.42） mIU/L] in obese group were significantly higher than those in the control group[（358.86 ±15.32） g,（3.61 ±0.45）%,（7.22 ±2.06） mIU/L] （all P〈0.05）,but the levels of the parameters in blueberry anthocyanidin group [（431.38 ± 13.10） g,（4.30 ± 0.37） %,（9.81 ± 0.98）mIU/L] showed no significant changes compared with those in obese group（all P 〉 0.05）.The level of HOMA-IR index was positively correlated with serum glucose,TC,TG,LDL,TNF-α,IL-6,and hs-CRP （r =0.733,0.668,0.602,0.607,0.683,0.728,0.784,0.907 and 0.743,all P 〈 0.01）.The level of HOMA-IR index was negatively correlated with HDL （r =-0.581,P 〈 0.01）.Conclusions Blueberry anthocyanidin enhances insulin sensitivity,which may be associated with the amelioration of the dyslipidemia and the less severe inflammation in high-fat-diet induced obese rats.