摘要
目的了解瘦素对肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导肝星状细胞(HSC)凋亡的影响及调控机制。方法 MTT比色法、流式细胞术检测瘦素对外源性TRAIL诱导HSC-T6细胞增殖和细胞凋亡的影响;采用Western印迹检测信号传导与转录激活因子3(STAT3)、磷酸化STAT3(pSTAT3)、Bcl-2。结果 TRAIL抑制HSC-T6细胞增殖、诱导HSC-T6细胞凋亡,而瘦素使TRAIL诱导的HSC-T6细胞凋亡明显减少(P<0.05);当TRAIL诱导HSC-T6细胞凋亡时加入瘦素,pSTAT3、Bcl-2表达上调。结论瘦素可以使外源性TRAIL诱导的HSC-T6细胞凋亡减少,可能与瘦素促使STAT3磷酸化,Bcl-2表达上调有关。
Objective To determine whether leptin protects rat hepatic stellate cells （HSC） HSC-T6 from TRAIL-induced apoptosis and its pathogenesis .Methods HSC-T6 cells were cultured and treated with TRAIL and leptin .The inhibition on proliferation of HSC-T6 cells was detected by 3-（4 ,5-dimethytthiazole-2-yl）-2 ,5-diphenyltetrazolium bromide （MTT） assay ,while apoptosis rate was detected by flow Cytometry .Western blot was performed to determine STAT3 ,pSTAT3 and Bcl-2 in HSC-T6 cells .Results Leptin increased cell proliferation of HSC-T6 cells treated with TRAIL and reduced apoptosis rate （P〈 0 .05） .Leptin pSTAT3 and Bcl-2 expression was increased by leptin using Western blot .Conclusion Leptin could reduce apoptosis of HSC-T6 cells treated with TRAIL effectively ,which was associated with high expressions of pSTAT 3 and Bcl-2 .
出处
《肝脏》
2014年第2期108-111,共4页
Chinese Hepatology
基金
黑龙江省教育厅资助项目(11551248)
关键词
瘦素
肿瘤坏死因子相关凋亡诱导配体
肝星状细胞
细胞凋亡
信号传导与转录激活因子3
Bcl-2
Leptin
Tumor necrosis factor-related apoptosis inducing ligand （ TRAIL）
Hepatic stellate cell
Apoptosis
Signal transducer and activator of transcription3（STAT3）
Bcl-2
