摘要
目的研究感染羊瘙痒因子139A的小鼠脑组织中自噬的活化及其机制。方法采用WesternBlot方法检测感染羊瘙痒因子139A的小鼠终末期脑组织匀浆中的AMPK、ULKl及其磷酸化水平和LC3II的表达,并对其进行定量分析。结果感染139A毒株的小鼠脑组织终末期脑组织中AMPK、ULKl及其磷酸化水平均表达升高。同时发现LC3II水平增加。结论AMPK-ULKl通路在139A感染的小鼠脑组织中活化并且有可能对自噬的激活起到了重要的作用。
Objective To investigate the activation of the autophagy and the signal transduction pathway in the scrapie 139A infected mice brain tissues. Methods The Western Blot was used to evaluate the expression level of AMPK, ULK1, p-AMPK, P-ULKI and LC3 II in the normal and scrapie 139A infected mice brain tissues. Results The expression levels of AMPK, ULK1 and their phosphorylated form p-AMPK (Thr172) , p-ULK1 (Ser555) are markedly up-regulated in the brains of the mice infected with scrapie agent 139A. Furthermore, the expression of LC3 II was also found increased in the brains of 139A infected mice, but not in normal mice. Conclusion AMPK-ULK1 pathway activated and contributed to autophagy in 139A infected mice brain.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2014年第2期126-128,共3页
Chinese Journal of Experimental and Clinical Virology