风疹病毒IgG抗体亲和力检测对近期感染诊断的应用价值

The value of rubella IgG avidity test in the diagnosis of recent rubella infection

目的评价风疹病毒IgG抗体亲和力检测对近期感染诊断的应用价值。方法对三个人群样本进行了风疹病毒IgG抗体亲和力检测，包括近期感染样本：BIOMEX公司风疹病毒血清转化盘RP-001、RP-014、RP—011共计48份；风疹病毒IgM阳性样本38份；风疹病毒既往感染（IgM阴性IgG阳性）样本978份。结果三个BIOMEX公司血清转化盘RP-001（至感染后第50天）、RP-014（至感染后第42天）、RP．011（至感染后第71天），检测均为低亲和力；38份风疹病毒IgM阳性样本中有24份检测为低亲和力，9份检测为高亲和力；978份既往感染人群样本，有963份检测为高亲和力，9份检测为低亲和力，6份检测为灰区。结论IgG抗体亲和力的检测对于区分近期感染和既往感染具有较高的诊断价值，对于IgM阳性的人群，其中约有25％检测为高亲和力，提示对该人群进行抗体亲和力的检测尤为重要。

Objective To evaluate the value of rubella IgG avidity test in the diagnosis of recent rubella infection. Methods We detected the rubella IgG avidity of three groups of serum samples： 48 acute infection samples from BIOMEX seroconversion panels RP-001, RP-014 and RP-011, 38 rubella IgM positive samples, 978 past infection samples （lgG positive IgM negative）. Results All the acute infection samples from BIOMEX seroconversion panels RP-001 （50 days after infection） , RP-O14 （42 clays after infection） , RP-011 （71 days after infection） show IgG low avidity. Of the 38 rubella IgM positive samples, 24 samples show IgG low avidity, 9 samples show IgG high avidity, 6 samples show grey zone. Of the 978 past infection samples, 963 samples show IgG high avidity, 9 samples show IgG low avidity, 6 samples show grey zone. Conclusions Rubella IgG avidity test can be used to rapidly distinguish between past and acute infection. Of the rubella IgM positive samples, about 25% show IgG high avidity, indicate past infection, so it is important to detect IgG avidity for this population.