摘要
目的探究白念珠菌生物膜形成过程中不同时期的锌簇转录因子TAC1与外排泵基因MDR1,CDR1,CDR2与白念珠菌生物膜耐药的关系。方法 96孔板培养白念珠菌生物膜,MTT法分别测定白念珠菌生物膜形成不同时期(4h,24h,48h)对氟康唑的敏感性。6孔板培养白念珠菌生物膜,分别在4h,24h,48h提取生物膜的总RNA,逆转录cDNA,RT-PCR检测TAC1,MDR1,CDR1,CDR2的基因表达量。结果白念珠菌在生物膜的形成过程中TAC1,MDR1,CDR1,CDR2的基因表达差异具统计学意义,经过Bonferroni多重比较,结果显示标准株00889 TAC1,MDR1,CDR1,CDR2早期基因表达量比中、晚期高。临床敏感株359 TAC1,MDR1,CDR1,CDR2中期基因表达量比晚期高。结论锌簇转录因子TAC1和外排泵基因可能在白念珠菌生物膜的早中期对氟康唑的耐药形成中发挥了一定作用。
Objective To explore the role of resistance-related genes encoding TAC1 MDR1 CDR1 CDR2 in the process of biofilm formation of Candida albicans. Methods Biofilms were formed and the susceptibility to fluconazole in different biofilm periods of C. albicans was respectively determined by MTr method. The total RNA was extracted from biofilms in different phase and then cDNA was synthesized. The expression of bio- film-related genes was detected by quantitative real-time PCR. Results The expression of TAC1, MDR1, CDR1 and CDR2 had meaningful differences in the process of candidal biofilm formation. Bonferroni test（P 〈 0. 05） revealed that the expression of TAC1, MDR1, CDR1 and CDR2 in the earlier phase was higher than that in the middle and late phases for the isolate 00889. For the clinical isolate 359 ,the expression of TAC1 MDR1, CDR1 and CDR2 in the middle phease was higer than that in the late phase. Conclusion It is likely that the TAC1 and efflux pump genes may play a role in the resistance to fluconazole of candidal biofilms in the early and middle phease.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2014年第5期467-471,共5页
The Chinese Journal of Dermatovenereology
