A1AR在低盐刺激小鼠肾素分泌中的可能作用

Potential role of A1AR in renin secretion stimulated by low salt diet in mice

目的观察腺苷A1型受体(A1AR)基因敲除小鼠的病理生理特点及A1AR在慢性低盐刺激肾素分泌中的作用。方法 C57BL/6J品系A1AR基因敲除杂合子(A1AR+/-)小鼠,经繁殖及基因鉴定后,杂合子子代小鼠用于扩大繁殖,A1AR+/+小鼠(n=13)及A1AR-/-小鼠(n=14)用于实验。观察两组小鼠生长发育、基本生理情况(血压、心率、肾功能和血尿电解质)、肾脏病理和免疫组化观察肾脏局部肾素表达和检测血浆醛固酮水平(A1AR+/+组n=5,A1AR-/-组n=5)。结果低盐饮食后,A1AR-/-小鼠肾皮质肾素表达明显增加(P<0.05),全身醛固酮水平和24 h尿钾排泄量显著高于A1AR+/+小鼠(P<0.05)。结论 A1AR可能参与了低盐刺激小鼠肾素分泌的过程。

Objective To observe the pathogenesis of adenosine A1 receptor （A1AR） gene knockout mice and to investigate the role of A1AR in renin secretion stimulated by low salt diet.Methods After A1AR＋/-mice of C57BL/6J mating,multiplication and gene identification,offspring mice were sampled.The differences of following items between A1AR＋/＋ （n =13）and A1AR-/-（n =14）mice were recorded：body weight,blood pressure,heart rate,renal function and electrolyte profile of serum and urine; Pathology features were microscopied after regular staining on kidney tissue samples.Renin expression at the kidney was stained by Immunohistochemistry.Plasma aldosterone level was also examined（A1AR＋/＋ group,n =5,A1AR-/-group n =5）.Results After low salt diet,much higher renin expression of the kidney cortex and plasma aldosterone level was observed in A1AR-/-mice,accompanied with increasing of 24-hour urine potassium excretion （P ＜ 0.05）.Conclusions A1 AR may mediate the renin secretion stimulated by low salt diet in mice.