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大鼠吸入氯气后肺组织Claudin-3蛋白及其mRNA、Occludin mRNA表达变化

Expression changes of Claudin-3 and Occludin in lungs of rats exposed to chlorine
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摘要 将40只SD雄性大鼠随机分为1个对照组和4个不同浓度的氯气染毒组,染毒10 min,6 h后行血气分析,取右肺中叶组织测湿干重比W/D和上叶组织行病理切片观察,Western blot法检测右肺副叶组织Claudin-3蛋白表达水平,RT-PCR法测右肺下叶组织Claudin-3 mRNA及Occludin mRNA相对表达。与对照组相比,(42.00±3.18)mg/m3及以上浓度氯气染毒组大鼠肺组织Claudin-3蛋白表达水平均有降低,差异有统计学意义(P<0.05);且与对照组相比,各染毒组大鼠肺组织Claudin-3 mRNA、Occludin mRNA表达水平也均有降低,差异有统计学意义(P<0.05)。提示大鼠吸入氯气后肺组织Claudin-3、Occludin表达减少可能是ALI肺水肿形成的机制之一。
作者 江俊康 覃江纯 许培培 瞿建华 王春 陈刚
机构地区 南通大学公共卫生学院
出处 《中国工业医学杂志》 CAS 北大核心 2014年第2期111-113,共3页 Chinese Journal of Industrial Medicine
关键词 氯气 CLAUDIN-3 OCCLUDIN 急性肺损伤
分类号 R994 [医药卫生—毒理学]
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参考文献16

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二级参考文献42

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中国工业医学杂志
2014年 第2期

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