附和唾液腺细胞的胶原支架体内移植实验

Experiment of Three-dimensional Collagen Scaffold with Salivary Gland Cells which Implanted in the SD Rats

目的:探讨附和大鼠唾液腺细胞(Salivary Gland Cells,SGCs)的三维胶原支架材料移植SD大鼠体内表达情况。方法:培养SD大鼠SGCs,增殖提纯、鉴定后将其接种到三维胶原支架材料上,植入SD大鼠皮下,连续观察3周检测SGCs表达情况。结果:实验组:SD大鼠生长良好,免疫组化显示SGCs连续3周均有表达,胶原支架材料3周时完全吸收。对照组:无SGCs的三维胶原支架材料植入SD大鼠皮下未见阳性细胞表达。结论:附和SGCs的三维胶原支架材料植入大鼠体内能够维持表达。

Objective： To investigate the expression of SD rats in vivo which had transplanted three--dimensional collagen scaffold with salivary gland cells （SGCs）. Methods： The SGCs of SD rats were cultured, proliferation puri- fied, identified, and then inoculated into three--dimensional collagen scaffold which implanted in SD rats subcutane- ously, and detected the expression of SGCs for 3 weeks continuously. Results： Experimental group： SD rats grew well. Immunohistochemistry showed that SGCs was expressed in three weeks consecutively, and collagen scaffold completely absorbed at three weeks. Control group： There was not the expression of positive cells in SD rats subcu- taneously which implanted the three--dimensional collagen scaffolds without SGCs. Conclusion： The three--dimen- sional collagen scaffold with SGCs could express continuously which implanted in the SD rats.