金叶连翘组培快繁技术研究

Study on Rapid Propagation Technique for Forsythia koreanna by Tissue Culture

为了建立金叶连翘组培快繁体系,采用金叶连翘新生枝梢为外植体,采用不同浓度激素处理,研究组培快繁过程中外植体分化、增殖、生根及相关因素对金叶连翘快繁的影响。结果表明:金叶连翘最佳的初代培养体系为MS+6-BA 3 mg·L-1+NAA 0.1 mg·L-1,增殖培养体系为MS+6-BA 2.5 mg·L-1+NAA0.1mg·L-1,生根培养体系为1/2MS+NAA 0.2mg·L-1或1/2MS+IBA 0.2mg·L-1。

In order to establish rapid propagation system for Forsythia koreanna ,taking new branch of Forsythia koreanna as explant, Hormone treatmens with different concentration were adopted, the effects of explant differentiation,proliferation,rooting and related factors on rapid propagation of Forsythia koreanna were studied. The results showed that the best primary culture medium was MS＋ 6-BA 3 mg·L-1 ＋NAA 0.1 mg·L-1 , and optimal medium for proliferation was MS＋6-BA 2.5 mg·L-1＋NAA 0.1mg·L-1 ,rooting medium was 1/2 MSWNAA 0, 2 mg·L-1 or 1/2MS＋IBA 0. 2 mg·L-1.