血浆和支气管肺泡灌洗液中脂连素和TXB2、6-keto-PGF1α水平与急性肺损伤关系的研究

Empirical study on the relationship between the levels of Adiponectin and TXB2,6-keto-PGF1αin serm and bronchoalveolar lavage fluid and acute lung injury

目的:观察急性肺损伤大鼠的血浆和支气管肺泡灌洗液中脂连素(APN)水平变化,并探讨其与急性肺损伤(ALI)程度的相关性。方法:将45只SD大鼠,随机分为实验组(根据观察时间分为4h组、12h组、24组、48h组)与正常对照组,实验组小鼠给与气管内雾化吸入内毒素(LPS)0.5mL/kg(LPS 200μg/mL),对照组气管内吸入生理盐水0.5mL/kg,观察各组大鼠光镜下的病理改变并进行病理评分;采用酶联免疫吸附法(ELISA)分别测定各组大鼠血浆及BALF均化处理后APN和TXB2、6-keto-PGF1α的含量,并对BALF中炎症细胞分类计数及白蛋白测定。结果:实验组中4h、12h组血浆和BALF中APN、6-keto-PGF1α水平低于正常对照组,且血浆中APN差异有统计学意义(P<0.01),血浆中APN水平与ALI的病理学评分呈较高的负相关(r=0.936,r2=0.877,P<0.01);4h、12h组血浆和BALF中TXB2水平高于正常对照组(P<0.05);24h、48h组中APN、TXB2及6-keto-PGF1α水平与对照组无差异(P>0.05)。结论:APN参与了ALI的发生发展过程且血浆APN水平可反映ALI程度。

Objective： To observe the variations of adiponectin in acute lung injury rats, and to investigate the relationship between the levels of APN and the degree of acute lung injury. Methods： 45 rat were randomly divided into 5 groups（each n= 9） ：normal control group （0. 5 mL/kg NS instilled into the trachea via a needle） and experimental groups（0. 5 mL/kg LPS instilled into the trachea via a needle）. Experimental groups were di- vided into 4h group, 12h group, 24h group, 48h group according to observation time. Rats were observed un- der light microscope for pathological changes and pathological scores. The concentrations of adiponectin and TXB2, 6-keto-PGFla in serum and bronchoalveolar lavage fluid （BALF） were determined by ELISA, and BALF was collected to assess the presence of poly morphonuclear neutrophils and albumin in BALF. Results： The concentrations of adiponectin and 6-keto-PGF1acute lung injury; bronchoalveolar lavage fluid; adiponectin; TXB2; 6 - keto - PGF1 α in serum and BALF in 4h group and 12h group were lower compared with those in normal control group, and the levels of APN in plasm have significant difference （P 〈 0.0l）. What＇s more, the APN levels in plasm of the rat with acute lung injury were negatively correlated with the lung injury pathological score （r = 0. 942 ,r2= 0. 884 ,P 〈 0. 01）. The levels of TXB2 in the plasm of 4h and 12h groups were higher than those of normal control group （P 〈 0. 05）. However, the levels of adiponectin and TXB2, 6-keto-PGF1acute lung injury; bronchoalveolar lavage fluid; adiponectin; TXB2; 6 - keto - PGF1 α in 24h and 48h groups had no difference from those of normal control group （P 〈 0.05）. Conculusion： Adiponectin palys a role in the development of acute lung injury, and adiponectin levels in plasm can reflect the degree of acute lung injury.