Survivin蛋白在卵巢癌组织中的表达及临床意义

Expression of survivin protein in epithelial ovarian cancer tissues and its significance

目的:运用组织芯片技术结合免疫组化的方法分析Survivin蛋白在正常卵巢、卵巢良性肿瘤、卵巢癌组织中的表达情况,探讨Survivin蛋白在卵巢癌的发生发展、浸润转移和化疗耐药中的作用,为以Survivin作为分子治疗靶点的抗肿瘤基因治疗提供理论依据。方法:运用组织芯片技术联合免疫组化法检测27例卵巢癌、20例正常卵巢组织、30例卵巢良性肿瘤中凋亡抑制基因Survivin的表达情况,分析其与卵巢癌临床病理特征的关系,并进行临床随访分析Survivin基因与卵巢癌化疗耐药的关系。结果:①Survivin蛋白在卵巢癌组织中的阳性表达显著高于卵巢良性肿瘤和正常卵巢,两两比较,Survivin在卵巢癌中的表达率与在正常卵巢组织及卵巢良性肿瘤中的表达率比较有差别,且差别有统计学意义(P<0.05);Survivin蛋白与卵巢癌的临床分期有显著相关性(P<0.05);Survivin蛋白表达与卵巢癌的组织类型、病理学分级、患者年龄、是否绝经、有无腹水形成无关(P>0.05)。②Survivin蛋白在卵巢癌化疗耐药组中高表达。结论:Survivin蛋白高表达在卵巢癌的发生发展中起重要作用,Survivin蛋白在卵巢癌以顺铂和紫杉醇为基础的化疗耐药中起重要作用。

Objective： Tissue Microarray （TMA） technology and immunohistochemical technique were used to detect the expression of Survivin in ovarian tumor tissues. The relationship among Survivin clinicopatho- logical features and drug resistance were analyzed to evaluate the biological behavior of ovarian cancer. This ex- periment was to discuss the effect of Survivin on the development, metastasis and the drug resistance of chemotherapy of ovarian cancer, so as to offer theoretical basis of antineoplastic therapy targeted to Survivin. Methods： Tissue microarray technology and immunohistochemical technique were used to detect the expression of Survivin in ovarian tumor tissues. The relationship among Survivin and Runx3 clinicopathological features and drug resistance were analyzed. Results： ①The positives rates of Survivin in ovarian cancer were higher than those in normal ovaries and in ovarian benign tumor （P 〈 0. 05）. The expression of Survivin was signifi- cantly correlated with the clinical stage of ovarian carcinoma （P 〈 0.05）. However, it was not correlated with histological type, pathological grade,age, menopause and ascites formation （P 〈 0.05）. ②Expression rate of Survivin in drug resistance group was higher than that in sensitive group. Conclusion： Survivin plays an impor- tant role in platinum--based and taxol chemotherapy.