摘要
目的 CST6基因编码cystatin M蛋白,体外构建CST6过表达载体,转染胃癌SGC-7901细胞株,观察cystatin M对其增殖和迁移能力的影响。方法实验分3组:pcDNA3.1(+)-CST6组、pcDNA3.1(+)组与未转染组;RTPCR验证稳转系SGC-7901/CST6细胞CST6RNA水平的表达;Western blot验证稳转系SGC-7901/CST6细胞CST6蛋白水平的表达;MTT实验检测实验组与对照组细胞的增殖能力;划痕实验检测实验组与对照组细胞的迁移能力。结果稳转系SGC-7901/CST6细胞稳定表达CST6基因mRNA和cystatin M蛋白;pcDNA3.1(+)-CST6组细胞增殖能力和迁移能力均较pcDNA3.1(+)组和未转染组细胞降低(P<0.05)。结论 Cystatin M能够抑制胃癌SGC-7901细胞株增殖和迁移能力。
Objective To encode the protein cystatin M with CST6 gene and construct a CST6-overexpression vector, and transfect it into the gastric cancer cells SGC-7901 in order to observe the effect of cystatin M on the proliferation and migration abilities of SGC-7901 cells. Methods There were three groups in the experiment： pcDNA3. 1（＋）-CST6 group, pcDNA3. 1 （＋） group and non-transfected group. RT-PCR and Western blot were used to identify the RNA expression of CST6 in SGC-7901/CST6 cells. The proliferation and migration abilities of the transfected cells were detected by MTT and cell wound healing assay, respectively. Results SGC-7901/CST6 cells stably expressed CST6 gene RNA and cystatin M protein. The proliferation and migration of pcDNA3.1 （＋）- CST6 group cells were reduced compared with those of cells in the other two groups （P〈0.05）. Conclusion Cystatin M can inhibit the proliferation and migration of SGC-7901 cells.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2014年第3期375-379,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
河南省国际科技合作计划项目(No.12430510009)
国家自然科学基金资助项目(No.81201955)~~
