赖氨大黄酸对快速老化小鼠SAMP 10肾组织TNF-α、IL-6、NF-κB表达的影响

Effects of rhein lysinate on the expressions of TNF-α,IL-6 and NF-κB in the kidney tissue of SAMP 10 mice

目的研究赖氨大黄酸(RHL)对快速老化小鼠(SAMP 10)肾组织肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)及核因子κB(NF-κB)蛋白表达调控的影响及其意义。方法选取7月龄SAMP 10小鼠18只,随机分为空白对照组及不同剂量的RHL组,另选抗快速老化小鼠(SAMR 1)6只作为青年对照,给药时间6周。采用HE染色观察肾脏组织病理学改变,免疫组化和Western blotting方法检测肾组织TNF-α、IL-6、NF-κB蛋白的表达。结果 RHL治疗对SAMP10小鼠体重没有显著影响(P>0.05)。与SAMR 1组比较,SAMP 10小鼠肾组织有节段性肾小球萎缩、硬化和炎细胞浸润,而RHL(25mg/kg和50mg/kg)治疗能阻断这一病理进程。另外,RHL治疗能抑制SAMP 10小鼠肾组织TNF-α、IL-6、NF-κB和磷酸化的NF-κB蛋白过度表达(P<0.05)。结论 RHL可抑制SAMP 10小鼠肾组织炎症反应,这可能是其发挥肾保护作用,从而起到抗衰老作用的机制之一。

Objective To investigate the effects of rhein lysinate （RHL） on the expressions of TNF-a, IL-6 and NF-kB in the kidney tissue of senescence accelerated mouse prone 10 （SAMP 10） mice. Methods We selected 18 male mice （SAMP 10） aged 7 months for the study and randomly divided them into blank control group and groups of different concentrations of RHL; six senescence accelerated mouse resistance 1 （SAMR 1） served as the young control group. After 6 weeks＇ treatment, HE staining was used to detect the pathological changes of the kidney. The expressions of TNF-α, IL-6 and NF-kB at the protein level were detected by immunohistochemistry and Western blotting. Results RHL treatment did not affect the body weight of SAMP 10 mice （P 〉 0. 05）. Compared with SAMR 1 mice, contracted and destroyed renal glomeruli and infiltration of mononuclear macrophages were observed in control SAMP10 mice. However, this pathological process was blocked by RHL （25 mg/kg and 50 mg/kg） treatments. In addition, the overexpressions of TNF-α, IL-6 and NF-kB and the phosphorylation of NF-kB in the kidney tissue of SAMP 10 mice could be inhibited by RHL treatments （P〈0.05）. Conclusion RHL inhibits the inflammatory reaction of the kidney tissue, which may be one of the mechanisms by which RHL exerts its kidney-protecting and anti-aging effects.