溃疡性结肠炎患者血清低氧诱导因子-1α的检测及意义

Detection and significance of serum hypoxia inducible factor-1α in ulcerative colitis patients

[目的]检测溃疡性结肠炎患者血清低氧诱导因子-1α(HIF-1α)的含量,探讨其在溃疡性结肠炎发病机制中的调控作用及其与患者病情活动性和严重性的关系。[方法]采用酶联免疫吸附试验(ELISA)定量检测13例缓解期(缓解期组)、47例活动期(活动期组)UC患者及10例健康体检者(正常对照组)血清中HIF-1α含量,并对活动期组患者行肠镜检查,评价病情轻重程度。[结果]活动期组HIF-1α含量[(73.21±28.65)ng/L]显著高于缓解期组[(44.54±14.75)ng/L]与正常对照组[(42.83±15.49)ng/L],P<0.05;缓解期组与正常对照组比较差异无统计学意义(P>0.05)。HIF-1α与病情活动性、病情分型及内镜表现分级呈正相关。[结论]HIF-1α作为一种转录因子可能在UC发病机制中充当重要角色,并可能作为评价UC患者病情活动性及严重程度的良好指标。

[Objective]To detect the serum hypoxia inducible factor-1 alpha（HIF-1α）in ulcerative colitis （UC）patients,explore its role in the pathogenesis of UC and its relationship with disease activity and se- verity. [Methods]Enzyme-linked immunosorbent assay（ELISA）was performed to detect the expression of HIF-1α in serum in 10 healthy controls,13 cases of remission UC and 47 cases of active UC ,the active UC patients underwent the colonoseopy to evaluate disease severity. [Results]The expression of HIF-1α in serum was significantly higher in active UC patients UC patients（[73.21 ± 28. 65]ng/L）than those in re- mission UC patients （[44. 54 ± 14. 75] ng/L） and control group （[42. 83 ± 15.49] ng/L）, P〈0.05 ; and no sig- nificant difference between remission UC patients and control group（P〉0. 05）. The level of HIF-la in serum was positively correlated with disease activity, disease severity and endoscopic grading. [Conclusion] HIF-1α, as a transcription factor, may play an important role in the pathogenesis of ulcerative colitis,and may serve as a good index to evaluate disease activity and severity in patients with UC.