孕鼠暴露低剂量邻苯二甲酸二己酯对仔鼠脂肪分布的影响

Effect of low dose di-2-ethylhexylphthalate exposure on fat distribution of offspring in pregnant mice

目的:探讨孕鼠暴露低剂量邻苯二甲酸二己酯(di-2-ethylhexylphthalate,DEHP)对仔鼠脂肪分布的影响及机制。方法:将C57BL/6J孕鼠分为2组,对照组喂饲基础饲料,DEHP组喂饲添加质量分数为0.06%DEHP的基础饲料,至仔鼠出生。9周后,处死仔鼠,切取附睾周围和腹股沟皮下脂肪组织,称质量;实时定量RT-PCR法检测Gpc4、Tbx15 mRNA表达。结果:DEHP组仔鼠附睾脂肪组织质量显著高于对照组(P<0.05);对照组和DEHP组仔鼠附睾脂肪组织Gpc4 mRNA表达均显著高于皮下脂肪组织(P<0.05),而Tbx15 mRNA表达则显著低于皮下脂肪组织(P<0.01);相比对照组,DEHP组仔鼠附睾脂肪组织Gpc4mRNA及皮下脂肪组织Tbx15 mRNA表达均显著增加(P<0.01)。结论:胚胎期低剂量DEHP暴露可能通过改变内脏及皮下Gpc4和Tbx15的表达,影响脂肪组织的分布。

Objective： To investigate the effect and mechanism of low dose di-2-ethylhexylphthalate （ DEHP ） exposure on fat distribution of offspring in pregnant mice .Methods： Pregnant C57BL/6J mice were divided into two groups.Mice in control group were fed with chow diet , and mice in DEHP group were fed with chow diet supplemented with 0.06%（ w/w ） DEHP till their offspring was born .Nine weeks after birth , the mice were sacrificed .Epididymal and inguinal subcutaneous adipose tissues were dissected and weighted .The expression of Gpc4 and Tbx15 mRNA was measured by real-time reverse transcription polymerase chain reaction （ RT-PCR ） method.Results：The weight of epididymal adipose tissues in DEHP group was significantly higher than that in control group （P〈0.05）.The expression of Gpc4 mRNA in epididymal adipose tissues was significantly higher＆amp;nbsp;than that in subcutaneous adipose tissues （ P〈0.05 ） , while the expression of Tbx 15 mRNA was significantly lower than that in subcutaneous adipose tissues （ P〈0.01 ） in both control and DEHP group .Compared with control group , Gpc4 mRNA expression in epididymal adipose tissues and Tbx 15 mRNA expression in subcutaneous adipose tissues were increased （ P〈0.01） .Conclusion：Low dose DEHP exposure in mice prenatal period perhaps affects fat distribution via modifying the expression of Gpc 4 and Tbx15 mRNA in visceral and subcutaneous adipose tissues.