大黄酚对铅中毒小鼠免疫功能的影响

Influences of chrysophanol on immune function of lead poisoning mice

目的研究大黄酚对铅中毒小鼠免疫功能的保护作用。方法采用连续8 d腹腔注射7 mg·kg-1醋酸铅造成铅中毒小鼠模型,大黄酚治疗14 d后检测脾脏和胸腺指数、白细胞计数、巨噬细胞吞噬功能、T、B淋巴细胞增殖能力,自然杀伤细胞(NK)活性。以酶联免疫吸附测定法(ELISA)测定铅中毒小鼠血清中细胞因子含量。结果连续8 d腹腔注射7 mg·kg-1醋酸铅造成铅中毒小鼠免疫能力下降,大黄酚可明显提高铅中毒小鼠的免疫能力,主要表现为连续腹腔注射大黄酚14 d治疗后,可不同程度改善铅中毒小鼠生长速度,明显提高铅中毒小鼠脏器指数和白细胞计数(P<0.05或P<0.01);大黄酚治疗组(1.0、10.0 mg·kg-1)可明显提高铅中毒小鼠B脾淋巴细胞增殖能力和T脾淋巴细胞增殖能力(P<0.01),大黄酚治疗组(1.0、10.0 mg·kg-1)可明显提高铅中毒巨噬细胞吞噬能力和NK细胞杀伤力(P<0.01),与溶媒组相比铅中毒模型组小鼠血清中IFN-γ、IL-2、IL-4和IL-10含量均明显降低(P<0.01);与模型组相比,大黄酚治疗组(1.0,10.0 mg·kg-1)小鼠血清中IFN-γ、IL-2、IL-4和IL-10均明显升高(P<0.01);与模型组相比,大黄酚治疗组(0.1 mg·kg-1)小鼠血清中IFN-γ、IL-2、IL-4含量未见明显变化(P>0.05),仅IL-10含量明显升高(P<0.05)。结论大黄酚可提高铅中毒小鼠免疫功能。

Aim To investigate the protective effects of chrysophanol（Chry） on immune function of lead poisoning mice. Methods The lead poisoning model was established by peritoneally injecting mice with 7 mg. kg-1 lead acetate every day for 8 days. After Chry was ip injected for 14 days, spleen and thymus index, the white blood cell count, T, B lymphocyte proliferation, phagocytic function of macrophages;natural killer cell （ NK cell） activity were detected. The concentrations of IFN-y , IL-2, IL-4, IL-10 in the lead poisoning mice serum were determined by enzyme-linked immunosorbent assay （ELISA）. Results Intraperitoneal injection of 7 mg . kg-1 lead acetate for 8 consecutive days could cause an immunity decline in lead poisoning mice, Chry could significantly improve the immunity of lead poisoning mice. Compared with model group, Chry significantly improved growth rate, viscera index and white blood cell count of lead poisoning mice at different extent （P 〈 0. 05 or P 〈 0. 01 ）. Chry （ 1. 0, 10.0 mg . kg - 1 ） significantly increased the B, T lymphocyte proliferation （P 〈 0. 01 ）and the phagocytosis of macrophage and NK cell activity （ P 〈 0.01 ）. Compared with the control group, the concentrations of IFN-y , IL-2, IL-4, IL-10 of lead poisoning mice serum were significantly reduced （ P 〈 0.01 ）. Compared with the model group, Chry （1.0,10. 0 mg . kg-1 ） significantly increased the concentrations of IFN- y, IL-2, IL-4, IL-10 （P 〈 0. 01 ） , while there were no significant changes in （0.1 mg . kg- 1 ） concentrations of IFN- y, IL-2, IL-4 in Chry （0.1 mg. kg-1） treatment group （P 〉 0. 05 ）, only IL-10 was significantly increased in Chry （0.1 mg. kg-1 ） treatment group （ P 〈 0.05 ）. Conclusion Chry can significantly improve the immune function of lead poisoning mice.