TLR4和NF-κB在肝内胆管结石相关的肝内胆管细胞癌中的表达及临床意义

Expression and clinical significance of NF-κB and TLR4 in intrahepatic lithiasis associated with intrahepatic cholangiocarcinoma

目的探讨Toll样受体4(TLR4)和核因子κB(NF-κB)在肝内胆管结石相关的肝内胆管细胞癌组织中的表达及其临床意义。方法收集110例胆管病理组织蜡块,包括40例肝内胆管结石相关的肝内胆管细胞癌组织、40例肝内胆管结石旁胆管组织和30例正常肝内胆管组织(肝血管瘤旁正常胆管组织或者肝外伤后切除肝组织的正常胆管组织),采用免疫组织化学(PV)法检测各组织中TLR4和NF-κB的表达情况并分析结果。结果肝内胆管结石相关的肝内胆管细胞癌组织中的TLR4和NF-κB表达量明显高于肝内胆管结石旁胆管组织和正常肝内胆管组织(P<0.05)。在肝内胆管结石相关的肝内胆管细胞癌组织中TLR4和NF-κB的表达呈正相关(r=0.709)。TLR4和NF-κB的表达与患者的性别、年龄、肿瘤分化程度及淋巴结转移的组间比较,差异无统计学意义(P>0.05)。结论 TLR4和NF-κB的表达与肝内胆管结石到肝内胆管结石相关的肝内胆管细胞癌的逐步发展具有密切相关性,且两者表达呈正相关。

Objective To investigate the expressions and clinical significance of NF-κB and TLR4 in intrahepatic lithiasis associated with intrahepatic cholangiocarcinoma. Methods Collect bile duct histopathologic wax blocks of liver tissues specimen of hepatectomy which were selected in department of pathology of the first affiliated hospital of Anhui medical university in recent 10 years, including 40 cases of intrahepatic bile duct calculi of intrahepatic bile duct carcinoma tissue,40 cases of intrahepatic biliary calculi by bile duct tissues and 30 cases of normal bile duct tissues（ normal bile duct tissues adjacent to liver hemangioma or liver trauma after resection of liver tissue of normal bile duct tissues） . Immune histochemical method （ PV） was tested in organizations in NF-κB and the expression of TLR4 and results were analyzed. Results The amount of NF-κB and TLR4 expression in intrahepatic biliary calcu-li of intrahepatic bile duct carcinoma tissue was significantly higher than that in the intrahepatic bile duct stones near bile duct tissues and normal bile duct tissue （ P〈0.05 ） . The expression of TLR4 and NF-κB in intrahepatic biliary calculi of intrahepatic bile duct carcinoma was correlative and presents positive correlation（r=0.709）. The comparison between groups of patients’age, sex, tumor differentiation, tumor invasion and lymph node metastasis that related the expression of TLR4 and NF-κB showed that there was no statistically significant difference （ P 〉0.05 ） . Conclusion The abnormal expression of TLR4 and NF-κB is closely associated with the gradual progress of intrahepatic bile duct stones complicated with intrahepatic bile duct carcinoma, and both are positively correla-ted.