耐盐绒毛白蜡特异性SCAR分子标记的鉴定

Identification of Salt Tolerant Fraxinus velutina SCAR Molecular Marker

该研究以耐盐型和盐敏感型绒毛白蜡及其F1代为材料,采用混合品系分析法进行RAPD分析。结果显示:在随机选取的150个10碱基随机引物中,仅有引物S20在耐盐基因池和盐敏感基因池间扩增出特异而可重复的592bp的多态性片段,命名为S20-592。获得的RAPD标记S20-592经克隆、测序、重新设计一对特异性引物转化成更稳定的SCAR标记。通过F1代个体验证,耐盐型个体均能扩增出此差异条带而盐敏感型个体中不能扩增出此差异条带,证明该SCAR标记的特异引物可用于耐盐绒毛白蜡物种的快速分子鉴定。

In this experiment, the resistant plant, sensitive plant and the F1 generation were used for identifi- cation of the molecular markers linked to salting tolerance gene of Fraxinus velutina by RAPD-BLA. The result indicated that 150 decamer random primers were used to amplify the DNA pools from the resistant plants and sensitive plants,only primer S20 could amplify a 592 bp specific fragment,and named S20-592. SCAR marker was developed from the RAPD marker S20-592 by cloning, sequencing and designing one pair of primers and the SCAR marker only presented in the individuals of the resistant plants. The fragment ex- isted in the resistant plants and F1 population,it was identification of salt tolerance F. velutina species. indicated that the SCAR primer can be used for rapid identification of salt tolerance F. velutina species.