皮肤鳞状细胞癌中Survivin，Caspase-3，COX-2的表达及临床意义

Expression of Survivin, Casepase-3 and COX-2 in squamous cell carcinoma and its clinical significance

目的探讨皮肤鳞状细胞癌及癌旁正常皮肤中Survivin，Caspase-3和COX-2的表达及临床意义。方法采用免疫组织化学法和荧光实时定量PCR，检测41例鳞状细胞癌及10例癌旁正常皮肤组织中Survivin，Caspase-3和COX-2蛋白及mRNA表达情况。结果癌旁正常皮肤中Survivin，Caspase-3和COX-2蛋白的阳性表达率分别是0、90．00％、10．00％，鳞状细胞癌中阳性表达率分别是68．29％、29．27％、73．17％。与癌旁正常皮肤相比，鳞状细胞癌中Survivin，Caspase-3和COX-2mRNA相对表达量为11．21、0．13、9．85，差异有显著的统计学意义。经相关性分析，鳞状细胞癌中Survivin与Caspase-3蛋白表达呈负相关（X2=11．994，P=0．001，R==0．598）；Survivin和COX-2蛋白表达呈正相关（X2=14．415，P=0，R=0．652）。结论Survivin，Caspase-3和COX-2蛋白可能参与皮肤鳞状细胞癌的形成；Survivin和COX-2蛋白协同促进鳞状细胞癌的发展；增加Caspase-3和／或减少Survivin、COX-2蛋白表达的靶向治疗，有望成为阻止皮肤鳞状细胞癌形成、发展的新途径。

Objective To explore the expression of Survivin, Caspase-3 and COX-2 in para-carcinoma normal skin and sqnamous cell carcinoma （SCC）. Methods The expression of Survivin, Caspase-3 and COX-2 in 41 cases of SCC and in 10 cases of para-earcinoma normal skin were detected by fluorescent quatititive PCR （FQ-PCR） and immunological histological chemistry （IHC）, respectively. Results The positive rates of Survivin, Caspase-3 and COX-2 in para-carcinoma normal skin were 0, 90.00% , 10. 00% , respectively. The positive rates of Survivin, Caspase-3 and COX-2 in SCC were 68.29 %, 29.27%, 73.17%, respectively. Com- pared with para-carcinoma normal skin, the relative expression of Survivin, Caspase-3 and COX-2 mRNA in SCC were 11.21, 0. 13, and 9.85, respectively. The results showed statistically significance. A negative correlation was found between the expression of Survivin and Caspase-3 proteins （X2 = 11. 994, P= 0.001, R = -0. 598） in SCC, and there was a highly positive correlation between Survivin and COX-2 protein expression （ X2 = 14. 415, P = O, R = O. 652）. Conclusion Survivin, Caspase-3 and COX-2 protein might be involved in the formation of SCC. Survivin and COX-2 proteins may cooperate to promote the progress of SCC. Target treatment of increase the expression of Caspase-3 and/or decrease the expression of Survivin and COX-2 might be possible to prevent the formation of SCC.