大黄素对肝纤维化大鼠肝脏氧化应激的影响

Effect of Emodin on the Oxidative Stress in the Liver of Hepatic Fibrosis Rats

目的:探讨大黄素对肝纤维化大鼠肝脏氧化应激的影响。方法:采用复合致病因素法建立肝纤维化大鼠模型并以大黄素(40mg/kg,每日灌胃)进行干预。4周末取材,行血浆肝功能及肝纤维化4项指标检测;观察肝组织病理学变化、检测肝组织匀浆中肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)、一氧化氮(NO)、过氧亚硝基阴离子(ONOO-)含量。结果:大鼠肝纤维化模型复制成功,模型组大鼠肝组织匀浆TNF-α、MDA、NO、ONOO-含量明显增加;大黄素干预组肝脏病理性损伤较模型组明显减轻,肝组织TNF-α、MDA、NO、ONOO-含量明显降低。结论:该种剂量大黄素可以通过降低肝脏的氧化应激水平,对肝脏纤维化的形成发挥一定的保护作用。

Objective：To explore the protective effect of emodin on oxidative stress in liver of hepatic fibrosis rats.Methods：The hepatic fibrosis rat model was established with multiple pathogenic factors and treated with emodin （40 mg/kg）for 4 weeks.The biochemistry indexes of ET,AST,ALT,TB,TC,TG and hepatic fibrosis indexes HA,LN,Ⅳ-C,PCⅢ were detected;The histopathological changes of hepatic tissues were observed and the contents of TNF-α、MDA、NO、ONOO-in hepatic tissues were detected.Results：The rat hepatic models were established successfully.In model group：the contents of TNF-α、MDA、NO、ONOO-in hepatic tissues increased significantly.In emodin group：Pathological inj ury of hepatic tissues was alleviated;the contents of TNF-α、MDA、NO、ONOO- decreased obviously. Conclusion：Emodin can reduce oxidative stress in liver and has a protective effect on hepatic firosis rats.