甜菜碱对肝纤维化大鼠细菌易位的干预作用

The Interventional Effect of Betaine on Bacterial Translocation in Rats with Hepatic Fibrosis

目的:探讨甜菜碱对肝纤维化大鼠细菌易位的干预作用。方法:随机将SD大鼠分为4组:正常对照组(N),甜菜碱干预的正常对照组(NB),肝纤维化模型组(M),甜菜碱干预的肝纤维化模型组(MB)。于4周末检测各组动物脏器指数与细菌易位情况。结果:大鼠肝纤维化模型复制成功。M组动物体重明显下降,与N组相比差异性显著(t=13.849,P=0.000,P<0.05);MB组动物较M组体重略有回升;与N组相比M组动物肝脏指数(t=-6.934,P=0.000,P<0.05)、肺脏指数(t=-7.061,P=0.000,P<0.05)、心脏指数(t=-3.873,P=0.004,P<0.05)、脑指数(t=-7.622,P=0.000,P<0.05)均显著升高;MB组动物较M组肝脏指数、脑指数有回降趋势。与N组相比,M组发生细菌易位的大鼠总数显著升高(χ2=6.000,P=0.014,P<0.05);与M组相比,MB组发生细菌易位的大鼠总数显著降低(χ2=4.000,P=0.046,P<0.05)。结论:4周肝纤维化动物的细菌易位以淋巴管道转位为主,甜菜碱可在一定程度上降低细菌易位的发生,从而发挥对肝脏的保护作用。

Objective：To explore the interventional effect of betaine on bacterial translocation in rats with hepatic fibrosis.Methods：Twenty-four male SD rats were randomly divided into four groups of six animals per group.Two groups were normal control group（N group and NB group）,and the other two were hepatic fibrosis models （M group and MB group）.In each condition,one group of rats was intragastricly administered with 1 000 mg/kg body weight betaine once daily for 4 weeks（NB or MB group）,whereas the other was treated with an equal volume of water（N or M group）.At the end of 4 weeks,the index of organs,and the bacterial translocation were assessed on blood,mesenteric lymph nodes,liver,spleen,kidney,heart,lungs and brain tissue.Results：The animal model of hepatic fibrosis was successfully established.The weight of M group was found to be significantly lower compared with N group（t=13.849,P=0.000,P〈0.05）,and the weight of MB group was slightly increased compared with M group.The organ index of liver（t=-6.934,P=0.000,P〈0.05）,heart（t=-3.873,P=0.004,P〈0.05）,lungs（t=-7.061,P=0.000,P〈0.05）and brain tissue（t=-7.622,P=0.000,P〈0.05）was significantly higher in M group than N group,and the organ index of liver and brain tissue demonstrated a back down trend in MB group compared with that of M group.The bacterial translocation in M group was significantly higher than that in N group（χ2=6.000, P=0.014,P〈0.05）,and the bacterial translocation was lower in MB group than that in M group（χ2=4.000,P=0.046,P〈0.05）.Con-clusion：The bacterial translocation is mainly transferred by lymphatic vessels in rats with hepatic fibrosis induced for 4 weeks.Betaine can decrease the occurrence of bacterial translocation in rats with hepatic fibrosis to some degree,and it has a protective effect on liver inj ury in rats with hepatic fibrosis.