3种免疫调节性寡聚脱氧核苷酸佐剂体外免疫活性的比较

In vitro immune activities of three kinds of immune regulatory oligodeoxynucleotide adjuvant

目的比较用于乙型肝炎疫苗的3种寡聚脱氧核苷酸(CpG-oligodeoxynueleotide,CpG ODN)佐剂体外的免疫活性。方法分别将CpG ODN 684(沃森公司)、CpG ODN 1018(Dynavax公司)和CpG ODN 7909(Coley公司)序列体外刺激免疫人外周血单个核细胞(peripheral blood mononuclear cell,PBMC)和人树突状细胞系GEN,采用氚-胸腺嘧啶核苷(3H-TdR)渗入法检测人PBMC增殖活力,ELISA法检测GEN细胞中IL-6和IFNα水平。结果 CpG ODN 7909、CpG ODN 1018和CpG ODN 684诱导人PBMC的增殖水平明显高于未刺激的对照组,差异有统计学意义(P<0.000 1),其中CpG ODN 684和CpG ODN 7909序列诱导人PBMC的增殖水平相似,略高于CpG ODN 1018序列;3种CpG ODN在体外对免疫细胞均具有明显的活化作用,其中CpG ODN 684和CpG ODN 7909序列体外活化免疫细胞产生的IL-6和IFNα水平相似,但高于CpG ODN 1018序列。结论沃森公司CpG ODN 684序列体外活化免疫细胞功能与Coley公司CpG ODN 7909序列相似,略优于Dynavax公司CpG ODN 1018序列。

Objective To compare the in vitro immune activities of three kinds of immune regulatory CpG oligodeoxynucleotide （CpG ODN） adjuvant. Methods Peripheral blood mononuclear cells （PBMCs） and human dendritic cell line GEN were stimulated with three kinds of CpG ODN, i.e. CpG ODN 684 （Walvax）, CpG ODN 1018 （Dynavax） and CpG ODN 7909 （Coley） in vitro separately. The proliferation activity of PBMCs were determined by 3H-TdR incorporation method, while the IL-6 and IFNct levels in GEN cells by ELISA. Results The proliferation levels of PBMCs stimulated with the three kinds of CpG ODN were significantly higher than those unstimulated （P 〈 0. 000 1 ）, of which those stimulated with CpG ODN 684 and CpG ODN 7909 were similar and were significantly higher than that with CpG ODN 1018. All the three kinds of CpG ODN adjuvant showed significantly activated the immune cells in vitro. However, the I1-6 and IFNcL levels in immune cells activated with CpG ODN 684 and CpG ODN 7909 were similar, which were higher than those with CpG ODN 1018. Conclusion CpG ODN 684 （Walvax） was similar to CpG ODN 7909 （Coley）, while was superior to CpG ODN 1018, in activation of immune cell function in vitro.