治疗性单克隆抗体表达载体的设计与优化策略

Strategy for design and optimization of expression vector for therapeutic monoclonal antibody

治疗性单克隆抗体的市场需求日益增长,因此如何分离得到高产的稳定表达细胞株及缩短研发过程成为此类研究的热点。在整个治疗性单克隆抗体的研发过程中,哺乳动物细胞表达载体的设计及其优化作为第一步就显得尤为重要。本文从基因转录水平的作用元件、作用方式、基因扩增与筛选标签及位置效应与整合位点的优化对治疗性单克隆抗体表达载体的设计与优化策略作一综述。

With the increasing needs for therapeutic monoclonal antibodies in market, the study in this field has focused on how to obtain a high yield and stable mammalian cell strain, which is mainly challenged by shortening the development process so as to obtain high yield and stable expression cell line. In the whole development process of therapeutic monoclonal antibodies, the design and optimization of mammalian cell expression vector as the first step play a particularly important role. This paper reviews the strategy for design and optimization of expression vector for therapeutic monoclonal antibody, including action element and mode at gene transcription level, label of gene amplification and screening, position effect as well as optimization of integration site.