摘要
目的研究沙参属药用植物荠苨中多糖成分的提取方法并测定其含量,为进一步开发利用提供可靠依据。方法采用正交设计法考察提取温度、时间、次数、料液比4个指标对多糖提取率及含量的影响;多糖含量采用苯酚-硫酸法测定。结果最佳提取条件为温度60℃、时间3h、次数1次、料液比1:30。最佳条件下,多糖提取率达18.17%,多糖含量为87.39%。结论本法操作步骤简单易行,荠苨多糖含量较高。
Objective To optimize the extraction progress and content determination of polysaccharide of Adenophora trachelioides Maxim.Methods Taking extraction ratio and content of polysaccharide as indexes,with temperature, extraction time,times of extraction,rate of solid to solution as factors ,extraction progress was optimized by orthogonal test.Results The optimum extraction technique was as follows：solid-solution ratio at 1：30,60℃,extracted for 3 hours,extracting one time.At the optimum condition,extraction ratio of Adenophora trachelioides maxim.reached 18.17% and the content of polysaccharide was 87.39%.Conclusion The optimal extraction technology was simple and feasible ,content ofpolysaccharide was high.
出处
《现代中药研究与实践》
CAS
2014年第2期50-52,共3页
Research and Practice on Chinese Medicines
关键词
荠苊
多糖
提取
含量
Adenophora trachelioides Maxim
polysaccharide
extraction
content
