蒙药手掌参-37味丸对亚急性衰老模型小鼠血清SOD、CAT、GSH-Px活性和MDA含量的影响

The Influence of Shouzhangshen-37 Pill of Mongolian Medicine on Serum SOD,CAT, GSH-Px Activity and MDA Content in Sub-Acute Aging Model Mice

目的:研究蒙药手掌参-37味丸对亚急性衰老模型小鼠血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)含量。方法:120只ICR雌性小鼠按体重随机分为6组:正常对照组、衰老模型组、维生素E组、蒙药手掌参-37味丸高、中、低剂量给药组。除正常对照组外,其余各组均采用颈背部皮下注射D-半乳糖,120 mg·kg-1,1次/d,续7周,建立亚急性衰老模型小鼠。同时蒙药手掌参-37味丸高、中、低剂量给药组小鼠灌胃0.5 mL/只(2.4、1.2、0.6 g·kg-1,生理盐水配制)手掌参-37味丸,维生素E组小鼠灌胃等体积(38.9 mg·kg-1,生理盐水配制)维生素E。7周后处死小鼠,摘眼球取血,检测血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)含量。结果:与正常组比较,模型组小鼠血清中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶的活性降低(P<0.01,P<0.05),丙二醛含量增多(P<0.01)。与模型组比较,蒙药手掌参-37味丸各给药组小鼠学血清中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶的活性增强(P<0.01,P<0.05),丙二醛含量降低(P<0.01,P<0.05)。结论:药手掌参-37味丸对亚急衰老模型小鼠具有抗衰老作用,其作用机制与提高小鼠的血清抗氧化酶的活性,降低丙二醛含量有关,从而增强小鼠抗氧化能力和免疫功能。

Objective： To study the influence of Shouzhangshen-37 pill of Mongolian medicine on serum SOD,CAT, GSH- Px activity and MDA content in sub-acute aging model mice. Methods： The 120 ICR female mice were categorized into six groups randomly： normal control group, model groups of aging, vitamin E group, high, middle and low doses of Shouzhangshen-37 pill of Mongolian medicine groups. Except the normal control group, all the other groups were injected D- galactose（120 mg·kg-1） once a day, lasting 7 weeks, to establishe sub-acute aging model mice. The high, middle and low doses of Shouzhangshen-37 pill of Mongolian medicine groups were intragastric administered Shouzhangshen-37 pill respectively for 0.5 mL each mouse （2.4, 1.2, 0.6 mg·kg-1 prepared with normal saline）; vitamin E groups were intragastric administered vitamin E same volume （38.9 mg·kg-1, prepared with normal saline）, for seven weeks. The mice were killed in 7 weeks; with the eyeball blood, the content of serum＇s superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GSH-Px） activity and malondialdehyde （MDA） were measured. Results： Compared with normal control group, the activity of SOD, CAT and GSH-Px in serum decreased（P〈0.01, P〈0.05） in model group, and the content of MDA in serum of model group increased（P〈0.01）. Compared with model groups, the activity of SOD, CAT and GSH-Px in serum increased （P〈0.01, P〈0.05）, and the content of MDA in serum decreased（P〈0.01, P〈0.05）, in high, middle and low doses of Shouzhangshen-37 pill of Mongolian medicine groups. Conclusion： Shouzhangshen-37 pill of Mongolian medicine had anti-aging effect on sub-acute aging mice. The mechanism was to enhance antioxidant enzymes activity in brain tissue, decrease content of MDA in brain tissue, thereby enhance the ability of mouse anti-oxidation and immune functions.