溪黄草不同提取方法抗菌活性比较研究

Comparative Antibacterial Qctivity of Rabdosin serra (Maxim) Hara Extracted by Conventional and Supercritical Methods

目的:比较溪黄草不同提取方法的抗菌活性。方法:采用超临界CO2流体+乙醇作为夹带剂的提取方法获得了溪黄草超临界提取物,与乙醇提取物、水提物抗菌活性进行了比较。结果:体外和体内抗菌方法研究均显示溪黄草超临界提取物对金黄色葡萄球菌和绿脓杆菌的抑菌效果均高于EE和WE。结论:溪黄草应用超临界CO2萃取的SFE具有更好的抗菌活性,可能部分与其较高含量的二萜化合物和脂肪酸有关。

Objective： To compare antibacterial activity of Rabdosin serra （Maxim） Hara extracted by conventional and su- percritical methods. Methods： Supercritical carbon dioxide modified with ethanol was used to obtain the extract of Rabdosin serra（Maxim） Hara, namely SFE. The antibacterial activity of SFE was analyzed and compared with ethanol extract （EE） and water extract （WE）. Results： The results indicated that SFE showed better antibacterial activity than those obtained by ethanol and water extraction. Conclusion： The higher total diterpenes and lipid acids may be related to its higher antibacterial effects.