基于量子点的赭曲霉毒素AsFLISA检测技术研究

Study on sFLISA of Ochratoxin A Based on Quantum Dots Technique

建立了基于量子点标记技术的赭曲霉毒素A（Ochratoxin A，OTA）双抗夹心荧光免疫检测（sand-wich fluorescence -linked immunosorbent assay ，sFLISA）体系，包含多克隆包被抗体、生物素标记的多克隆检测抗体、量子点标记的链霉亲和素等，获得了sFLISA的最佳操作参数：包被抗体浓度2．5μg/mL，检测抗体稀释500倍，量子点标记链霉亲和素稀释100倍。该方法在OTA浓度3．125～125μg/L之间时，相对荧光强度和OTA浓度呈线性关系，回归方程为y＝0．0206x＋0．2018，R2＝0．9924；加标回收率在90．1％～110．0％之间，变异系数均小于10％，能较好地进行赭曲霉毒素A的定量检测。

The sandwich fluorescence -linked immunosorbent assay （ sFLISA） system was established to detect ochratoxin A （OTA）, which included coated antibody , detecting antibody （labeled by biotin）, strepta-vidin （ labeled by QDs ） and other elements .The optimal operating parameters were obtained：coated concen-tration was 2.5 μg/mL;dilution ratio of detecting antibody was 1∶500;dilution ratio of streptavidin （labeled by QDs） is 1∶100.There was a linear relationship between RFU and OTA concentration in the range of 3.125~125 μg/L, and the linear equation was y =0.0206x ＋0.2018 （R2 =0.9924）.The average recovery was between 90 .1%and 110 .0%, and the coefficient of variation was below 10%.This system could detect OTA correctly.