高效液相色谱法测定金钱菇多糖的单糖组成

Analysis of monosaccharide compositions of Lentinus edodes polysaccharide by HPLC

采用高效液相色谱法,测定金钱菇多糖的单糖组成。用超声辅助提取金钱菇多糖,通过1-苯基-3-甲基-5-吡唑酮(PMP)衍生水解后的单糖,高效液相色谱法检测衍生物。结果表明:金钱菇多糖由甘露糖(Man)、核糖(Rib)、鼠李糖(Rha)、葡萄糖(Glc)、半乳糖(Gal)、木糖(Xyl)组成,其摩尔为1.00∶0.90∶0.91∶28.03∶1.58∶0.11。该方法快速、简便、重现性好,可用于测定金钱菇多糖的单糖组成。

Monosaccharide composition in Lentinus edode polysaccharides was determined by high performance liquid chromatography（HPLC）. The L. edodes polysaccharide was extracted using ultrasonic-assisted extraction. Monosac- charide were derived by 1-phenyl-3-methyl-5-pyrazolone（PMP） and the PMP derivatives of monosaccharide were analyzed by HPLC. The results showed that L. edodes polysaccharide was composed of mannose, ribose, rhamnose, glucose,galactose and xylose and their molar ratio was about 1.00 ： 0.90 ： 0.91 ： 28.03 .＂ 1.58 ＂ 0.11. The method was rapid, simple and reproducible and could be used to determine monosaccharide compositions of L. edodes.