铁筷子提取物对肿瘤细胞增殖及COX-2 mRNA表达的抑制作用

Inhibitory effect of Helleborus thibetanus extracts on the proliferation of tumor cells and expression of COX-2 mRNA

研究不同铁筷子提取物对肿瘤细胞增殖及COX-2mRNA表达的抑制作用。以铁筷子醇总提取物(TKZ1)、正丁醇萃取部位(TKZ2)、乙酸乙酯萃取部位(TKZ3)分别作用于DU145、PC3、HeLa、HT-29、HepG2等肿瘤细胞,应用噻唑蓝实验(MTT法)计算其对细胞增殖的抑制作用,应用荧光定量PCR技术检测TKZ1、TKZ2、TKZ3处理后的各肿瘤细胞中COX-2mRNA的表达情况。结果表明:TKZ1、TKZ2、TKZ3均能显著抑制多种肿瘤细胞的增殖,与阴性对照组比较,其可以在mRNA水平上抑制COX-2的表达,且呈明显的量效关系。说明铁筷子提取物对体外肿瘤细胞的增殖具有显著的抑制作用,其抗瘤机制可能与抑制肿瘤细胞中COX-2mRNA的表达有关。

To investigate the inhibitory effect of extracts from Helleborus thibetanus on cell proliferation and expression of COX-2 mRNA, the inhibitory effects of the total ethanol extract （TKZ1）,n butanol fraction （TKZ2）,and ethyl acetate fraction （TKZ3） on DU145,PC3, HeLa, HT-29 and HepG2 tumor cell lines were examined by MTT assay, and the COX-2 mRNA expression levels were analyzed by quantitative PCR. The results showed that TKZ1, TKZ2 and TKZ3 could significantly inhibit the proliferation of tumor cell lines and suppress the expression of cox- 2 on the mRNA level in a dose-dependent manner compared with the control. All three samples from Helleborus thi- betanus （TKZ） could significantly inhibit the proliferation of tumor cells in vitro, and the mechanism might be related to the inhibition of the COX-2 mRNA expression in tumor ceils.