云锦杜鹃NRT基因序列的生物信息学分析

Bioinformatics analysis of NRT gene sequences in Rhododendron fortunei

通过转录组测序,获得在接种ERM真菌的云锦杜鹃苗根系中显著差异表达的基因,其中硝酸根转运蛋白(NRT)基因是硝态氮吸收转运的关键基因。利用生物信息学方法,分析云锦杜鹃根转录组的硝酸根转运蛋白(NRT)基因序列,对其推导的氨基酸的理化性质、亲水性/疏水性、跨膜结构、导肽、二级结构、高级结构进行预测,并对硝酸根转运蛋白的氨基酸做进化发育分析。为进一步了解NRT基因在云锦杜鹃接种苗根系氮素吸收的作用奠定了基础。

We used next generation sequencing technology to investigate the transcriptomes between inoculated roots and uninoculated roots of the Rhododendron fortunei and obtained many differentially expressed genes. In this paper, the nucleic acid sequences and amino acid sequences of nitrate transporters from R. fortunei, were analyzed by bioinformatics tools. Several parameters of these sequences,including sequences composition, physicochemical property, leader peptide, topological structure of transmembrane regions, hydrophobicity or hydrophilicity, secondary structures, functional domains and protein structures, were predicted. Phylogenetic tree was reconstructed for the nitrate transporters protein family. Provide the bioinformatics foundation to understand NRT gene＇s function in inoculated seedling roots.