蓝花喜盐鸢尾化学成分预试及薄层色谱分析

Chemical test and thin-layer chromatography analysis of Iris halophila var. sogdiana

探讨蓝花喜盐鸢尾(Iris halophila var.sogdiana)的化学成分,建立其各萃取部分指纹图谱。采用植物化学成分系统预试法、薄层色谱分析技术及平面色谱图像定量法对蓝花喜盐鸢尾化学成分进行分析。蓝花喜盐鸢尾含有生物碱、有机酸、酚类、鞣质、黄酮、蒽醌、皂甙、香豆素、挥发油及三萜类化合物,无强心甙、氰甙和脂肪族硝基化合物。石油醚、氯仿、乙酸乙酯和正丁醇萃取相中至少含有10、7、9和7种化合物,经与标准品对照,乙酸乙酯相中含有鸢尾苷;各萃取相中相对含量最高的斑点依次为3、7、5和2。蓝花喜盐鸢尾化学成分主要集中在石油醚和乙酸乙酯萃取部分。蓝花喜盐鸢尾含有丰富的活性成分,具有一定的药用价值。

For further investigating the chemical principals of Iris halophila var. sogdiana and gaining finger printing of all abstraction fraction, then providing theoretical bases to develop its medicinal value, phytochemistry composition preliminary systematic and special tests and thin layer chromatography （TLC） were applied to analyze the chemical principals. The results showed that the plant contained alkaloids, organic acid, phenols, tannin, flavonoids, anthraquinone, saponins, coumarin, volatile oils, triterpenes, et al. But no cardiac glycoside, cyanophoric glycoside and aliphatic nitro compounds were found; it showed that 10, 7, 9 and 7 kinds of principals were located in the four fractions after analyzed by TLC and tectorigenin was contained in acetic ether fraction when compared with proof sample. The silica gel plates were analyzed to determine the contents with thin layer chromatogram scanner, the results showed that Rf3, Rf7, Rf5, Rf2 points were the maximum content points in the four fractions respectively. The chemical principals of Iris halophila var. sogdiana were at most in petroleum benzene fraction and acetic ether fraction. There were affluent active constituents in Iris halophila vat. sogdiana, therefore it has fine medicinal value.