广东省蝙蝠体内汉城型汉坦病毒的检测初报

Primary report of Seoul Hantavirus detected in bats from Guangdong province

汉坦病毒是一种人兽共患病病原,通过RT-PCR扩增汉坦病毒M片段基因序列检测我国广东蝙蝠,首次在广东地区健康蝙蝠样品中扩增出长度约170 bp的汉坦病毒cDNA片段,测序证实为汉坦病毒的M片段序列,同源性达到99%以上。共检测了5个蝙蝠种类的74只蝙蝠的咽、肛拭子样品,阳性率为12.16%。说明蝙蝠也携带和传播汉坦病毒,是汉坦病毒的宿主动物。鉴于蝙蝠与人类关系越来越密切,提醒我们在畜牧业和种植业工作中要加强防范蝙蝠传播病毒给家养动物和人类。

Hantavirus is a kind of animal disease pathogen. Partial M gene sequences of Hantavirus were amplified by RT-PCR to detect bats in Guangdong province and about 170 bp eDNA fragments of Hantavirus in samples from healthy bats of Guangdong were obtained for the first time. By sequencing nucleotides, these sequences belonged to Hantaviruses and had more than 99% homology with M gene fragment. Oral swab and anal swab samples from 74 bats of 5 species were tested and the positive rate was 12.16%. Results showed that bats carried and spreaded Hantaviruses and were host animals of Hantaviruses. In view of the relationship between bats and humans becoming more and more close, it is important to prevent bats from transmitting viruses to domestic animals and humans in animal husbandry and planting industry.